[Degradation of acid blue 45 in a white-rot fungi reactor operated under non-sterile conditions].

To operate the white-rot fungal reactor under non-sterile conditions has important significance for its practical applications. Ozone (0.0144 mg/min) was selected as the bactericide to control the bacterial contamination in a white-rot fungal reactor. The control effect on bacteria, enzyme production, pH variation and decolorization of a recalcitrant dye (acid blue 45) in the reactor were studied during continuously operating the reactor. It was found that, the contamination bacteria were controlled at under a number of 1 x 10(5)-1 x 10(6) CFU/mL and the inactivation rate of contaminating bacteria in white-rot fungal reactor was close to 99%. The Phanerochaete chrysosporium continuously produced MnP with the maximum residual activity of 50 U/L and degraded the acid blue 45 with a rate of 40%-80%. At around pH 6, the system still had the capability to continuously produce MnP and degrade the acid blue 45. To keep the growth of white-rot fungi and production of MnP for a long-term operation is a next step point to be solved.