AI Article Synopsis
- The study addresses the challenge of detecting less abundant molecules in biological samples, where their signals can be masked by nonresonant background noise.
- A new method is introduced that allows scientists to measure the phase of oscillators within the sample, improving the ability to identify hidden molecules by using a specialized phase-preserving technique.
- This phase detection is similar to earlier microscopy techniques, expanding on the concepts introduced by Zernike in 1933 for better clarity in vibrational motion analysis.
Article Abstract
In biological samples the resonant coherent anti-Stokes Raman scattering signal of less abundant constituents can be overwhelmed by the nonresonant background, preventing detection of those molecules. We demonstrate a method to obtain the phase of the oscillators in the focal volume that allows discrimination of those hidden molecules. The phase is measured with respect to the local excitation fields using a cascaded phase-preserving chain. It is measured point-by-point and takes into account refractive index changes in the sample, phase curvature over the field-of-view, and interferometric instabilities. The detection of the phase of the vibrational motion can be regarded as a vibrational extension of the linear (refractive index) phase contrast microscopy introduced by Zernike around 1933.
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| http://dx.doi.org/10.1103/PhysRevLett.103.043905 | DOI Listing |
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