The global increase in the number of applications involving therapeutic plasmid DNA (pDNA) is creating a need for large amounts of highly stable and purified molecules. One of the main obstacles during the developmental stages of a new therapeutic DNA molecule involves tackling a wide array of structural instability events occurring in/with pDNA and therefore assuring its structural integrity. This review focuses on major instability determinants in pDNA. Their elimination could be considered an important step towards the design of safer and more efficient plasmid molecules. Particular emphasis is given to mutations triggered by the presence of repeated sequences, instability events occurring during plasmid intracellular routing, instability mediated by insertion sequences and host genome integration.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.tibtech.2009.06.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Background: Traumatic anterior shoulder dislocation is the most common type of joint dislocation, with an incidence of 11 to 29 per 100 000 persons per year. Controversy still surrounds the recommendations for treatment and the available procedures for surgical stabilization.
Methods: This review is based on pertinent publications (2014-2024) that were retrieved by a selective search in the PubMed and Google Scholar databases.
Comparative study regarding the stability of a proximal ulnar stump with or without distal oblique bundle reconstruction during the Sauvé‒Kapandji procedure: a finite-element analysis.
Front Bioeng Biotechnol
January 2025
Department of Orthopedics, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, China.
Background: The most common postoperative complication of the Sauvé‒Kapandji (S-K) procedure is proximal ulnar stump instability. The distal oblique bundle (DOB) is a stable tissue used to stabilize the distal radioulnar joint. This study created finite-element models of the distal oblique bundle (DOB) to determine its effect on the proximal ulnar stump instability encountered during the Sauvé‒Kapandji procedure.
View Article and Find Full Text PDFAtomic Insights into pH-Dependent and Water Permeation of mRNA-Lipid Nanoparticles.
Mol Pharm
January 2025
Research Center of the Ministry of Education for High Gravity Engineering and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
The exposure of mRNA to water is likely to contribute to the instability of RNA vaccines upon storage under nonfrozen conditions. Using atomistic molecular dynamics (MD) simulations, we investigated the pH-dependent structural transition and water penetration behavior of mRNA-lipid nanoparticles (LNPs) with the compositions of Moderna and Pfizer vaccines against COVID-19 in an aqueous solution. It was revealed that the ionizable lipid (IL) membranes of LNPs were extremely sensitive to pH, and the increased acidity could cause a rapid membrane collapse and hydration swelling of LNP, confirming the high releasing efficiency of both LNP vaccines.
View Article and Find Full Text PDFNKX6.3 modulation of mitotic dynamics and genomic stability in gastric carcinogenesis.
Cell Commun Signal
January 2025
Department of Pathology, Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591, South Korea.
Background: Gastric cancer remains a significant global health challenge, characterized by poor prognosis and high mortality rates. Mitotic integrity and genomic stability are crucial in maintaining cellular homeostasis and preventing tumorigenesis. The transcription factor NKX6.
View Article and Find Full Text PDFUnderstanding rangeland desertification through pastoralist perspectives using a grounded theory approach.
Sci Rep
January 2025
Researcher of Rural Development and Social Issues in the Field of Natural Resources and Agriculture, Gorgan, Iran.
Rangeland desertification risk has significantly increased due to the fragility of these ecosystems and the severity of degradation caused by climate instability and human activities over the last decade. This research focuses on identifying indicators of rangeland desertification risk using a qualitative grounded theory approach based on the perspectives of pastoralists in Kolijah and Qolaq-Borte, Golestan Province, Iran. The study population comprised regional pastoralists, with 15 experts selected through snowball sampling.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!