AI Article Synopsis
- A new densitometric method was developed to measure complex mixes of conjugated estrogens in raw materials and tablets.
- The procedure involves hydrolyzing sodium sulfate esters of various estrogens, extracting free estrogens and an internal standard (methyltestosterone), and separating them on TLC plates with a specific solvent mixture.
- The method allows for quick and accurate quantitative analysis by scanning estrogen spots at 280 nm, making it effective for quality control in pharmaceuticals.
Article Abstract
A densitometric method for determination of complex mixtures of conjugated oestrogens in raw material and tablets was developed. The proposed procedure comprised hydrolysis of sodium sulphate esters of oestrone, equilin, 17 alpha-oestradiol, equilenin, 17 alpha-dihydroequilin and 17 alpha-dihydroequilenin, the chloroform extraction of free oestrogens and methyltestosterone (internal standard) and separation on TLC plates using chloroform-cyclohexane-dioxane-triethylamine (4.5:4.1:0.6, v/v) as the solvent for development. Quantitative assay was achieved by direct scanning of the oestrogen spots at 280 nm. The proposed method is simple, rapid, reproducible and adequate to control the content of conjugated oestrogens in the raw material and in pharmaceutical preparations.
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| http://dx.doi.org/10.1016/0731-7085(90)80034-m | DOI Listing |
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