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Background: The pioneering work by Dr. Payne et al. in time-lapse cinematography for observation of the morphokinetic features of human embryos inspired us to develop a new in vitro culture system with high-resolution time-lapse cinematography (hR-TLC) back in 2001.
View Article and Find Full Text PDFDirect cleavage during the first mitosis is a sign of abnormal fertilization in cattle.
Theriogenology
April 2023
Department of Biological Production, Tokyo University of Agriculture and Technology, Tokyo, 183-8538, Japan. Electronic address:
Article Synopsis
- Direct cleavage is an abnormal cleavage process in which a zygote divides into three or more cells, more common in those with multiple pronuclei (multi-PN) and abnormal pronuclei migration.
- * In an experiment, they removed lipid droplets from bovine zygotes to better visualize pronuclei, finding a direct cleavage rate of 78.6% in multi-PN zygotes, compared to 0.0% in zygotes with one pronucleus.
- * The study revealed that zygotes with non-apposed pronuclei had a much higher direct cleavage rate (100% for multi-PN) than those with apposed pronuclei, highlighting significant differences in cleavage behavior based on pron
Effects of oxygen tension and humidity on the preimplantation development of mouse embryos produced by in vitro fertilization: analysis using a non-humidifying incubator with time-lapse cinematography.
Exp Anim
August 2022
Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada, Obihiro, Hokkaido 080-8555, Japan.
To examine the effects of oxygen tension and humidity on early embryonic development, the preimplantation development of mouse embryos produced by in vitro fertilization was assessed by time-lapse cinematography to evaluate morphokinetic development with higher precision. Zygotes were produced from spermatozoa and oocytes from ICR mice and cultured in KSOM under low or high oxygen tension in a non-humidified incubator with time-lapse cinematography (CCM-iBIS). The developmental rates of embryos to the 4-cell and blastocyst stages under lower oxygen tension in CCM-iBIS were significantly higher than those under higher oxygen tension in CCM-iBIS.
View Article and Find Full Text PDFMorphokinetic analysis of pronuclei using time-lapse cinematography in bovine zygotes.
Theriogenology
May 2021
Department of Biological Production, Tokyo University of Agriculture and Technology, Tokyo, 183-8538, Japan. Electronic address:
The morphokinetics of pronuclei (PN) are considered crucial factors affecting embryogenesis in mammals. Whereas, since bovine zygotes contain a large number of cytosolic lipid droplets, detailed observation of PN has not been performed. In this study, we visualized PN using time-lapse cinematography (TLC) with light microscopy for the first time in delipidated bovine zygotes.
View Article and Find Full Text PDFDevelopmental kinetics and viability of bovine embryos produced in vitro with sex-sorted semen.
Theriogenology
February 2021
Research and Development Group, Zen-noh Embryo Transfer Center, Kamishihoro, Hokkaido, Japan.
The present study aimed to elucidate the developmental kinetics, growth potential, and viability of bovine embryos produced in vitro with sexed semen. Bovine oocytes were fertilized in vitro using unsorted and X-sorted semen from the same Holstein bulls, and the kinetics of in vitro development were continuously monitored for 10 d through time-lapse cinematography. The blastocyst formation rate was lower in the X-sorted group than in the unsorted group (P < 0.
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