AI Article Synopsis
- Birefringent particles will rotate when they are caught in elliptically polarized light, which is influenced by the type of microscope used.
- When using an infinity corrected oil-immersion objective, the rotation of these particles is hindered by spherical aberration at the interface between glass and water.
- By employing a finite-distance-corrected objective, it’s possible to optimize the trapping and rotation of birefringent particles, especially improving the rotation rates at deeper depths beyond the coverslip.
Article Abstract
Birefringent particles rotate when trapped in elliptically polarized light. When an infinity corrected oil-immersion objective is used for trapping, rotation of birefringent particles in optical tweezers based on an infinity optical microscope is affected by the spherical aberration at the glass-water interface. The maximum rotation rate of birefringent particles occurs close to the coverslip, and the rotation rate decreases dramatically as the trapped depth increases. We experimentally demonstrate that spherical aberration can be compensated by using a finite-distance-corrected objective to trap and rotate the birefringent particles. It is found that the trapped depth corresponding to the maximum rotation rate is 50 microm, and the rotation rates at deep trapped depths are improved.
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| http://dx.doi.org/10.1364/ao.48.004397 | DOI Listing |
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