The use of tissue softening agents to improve microtomy of keratotic tissues is employed widely. Many of these softeners contain hazardous constituents such as phenol. In this study, the use of non-ionic surfactants or non-toxic ingredients are investigated with the aim of creating a new softening agent. The new agent should be more effective in facilitating the sectioning of hardened tissue while reducing toxicity and complications associated with sectioning hard tissue compared to a commercially available phenol-based formulation. Four formulations are compared against the commercial product for their capability to section routinely processed paraffin-embedded tissue under standard operating procedure parameters. The trial formulations were shown to be fast acting and enabled improved serial sectioning of hard keratotic tissue in nearly all the cases tested. There was no evidence of adverse staining using either tinctorial or immunohistochemical methods. The new formulations had advantages over the commercially available solutions, improving on the number and quality of sections attainable from the tissue blocks, as well as offering a composition less toxic than phenol-based products.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/09674845.2009.11730246 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
First Report of Causing Fruit Rot on Fig in China.
Plant Dis
January 2025
Kashi, Xinjiang, China, China;
Fig (Ficus carica L.) holds economic significance in Atushi, Xinjiang, but as fig cultivation expands, disease prevalence has risen. In July 2024, approximately 22% of harvested fig (cv.
View Article and Find Full Text PDFGlobal insights and advances in edible coatings or films toward quality maintenance and reduced postharvest losses of fruit and vegetables: An updated review.
Compr Rev Food Sci Food Saf
January 2025
Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Postharvest Preservation and Processing of Vegetables (Co-Construction by Ministry and Province), Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, Food Science Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Transitioning to safe, nonthermal, and edible strategies for maintaining fruit and vegetable (F&V) quality, reducing postharvest losses (up to 55% annually), and ensuring food security requires extensive research and innovation in postharvest technologies. This review aims to provide an updated understanding of edible coatings or films (ECF), focusing on their role in reducing F&V postharvest losses, based on data from the last 40 years retrieved from the Web of Science database. The global ECF research network is represented by publication trends, majorly researched F&V, key research areas, influential and emerging authors, and global research ranking.
View Article and Find Full Text PDFInterface-Engineered Manufacturing of Gradient Wood with Heterogeneous Structure for Lightweight and Sustainable Structural Materials.
Nano Lett
January 2025
Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China.
Developing sustainable structural materials to replace traditional carbon-intensive structural materials fundamentally reshapes the concept of circular development. Herein, we propose an interface engineering strategy that utilizes water as a liquid medium to replace the residual air within natural wood. This approach minimizes the absorption of water-based softening agents by microcapillary channels of wood, enabling the controlled softening of the cell walls.
View Article and Find Full Text PDFFirst Report of Causing Soft Rot in Bananas in Ecuador.
Plant Dis
December 2024
Universidad de las Fuerzas Armadas, Ciencias de la Vida y la Agricultura, Sangolqui, Pichincha, Ecuador;
Bananas are Ecuador's second largest non-oil export product, and the quality of its fruit has established a strong presence in international markets. One-third of the world's banana exports originate from Ecuador. The Ecuadorian banana market is diversified, exporting fruit to various countries worldwide, making it a vital socio-economic and food security support for the country.
View Article and Find Full Text PDFMethyl jasmonate enabled maintained the postharvest flavor quality of ginger (Zingiber officinale roscoe) by reducing the loss of terpene volatile compounds.
Food Chem
March 2025
Hubei key Laboratory of Spices & Horticultural Plant Germplasm Innovation & Utilization, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China; Spice Crops Research Institute, College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China; College of Smart Agriculture /Institute of Special Plants, Chongqing University of Arts and Sciences, Yongchuan 402160, Chongqing, China. Electronic address:
Ginger, as a globally vital medicinal and food homologous crop, plays an irreplaceable role in human diet and healthcare. However, during the storage of ginger, the decline of physical properties and degradation of volatile flavor quality have emerged as an industrial concern that severely restricts the market value of the product. MeJA plays an essential role in extending fruit shelf life and regulate the synthesis of volatiles in horticultural products, yet its application in ginger remains unreported.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!