AI Article Synopsis
- The study aimed to create an effective chitosan film for drug sustained-release systems, focusing on its release properties, degradation, and potential toxicity to osteoblasts.
- Researchers optimized chitosan film preparation conditions through orthogonal experiments, which included testing drug release rates with Coomassie brilliant blue G250 and assessing degradation with lysozyme in buffer.
- Results showed that the optimal conditions produced a chitosan film that released 33.13% of bovine serum albumin within 8 days, had a reasonable degradation rate, and exhibited low cytotoxicity, making it suitable for biomedical applications.
Article Abstract
Objective: To develop a best chitosan film for using as a drug sustained-release system through the evaluation of the sustained-release property, degradation property, and cytotoxicity to osteoblast.
Methods: Orthogonal experiments were designed to determine the best combination of chitosan film preparations. Drug release rate was determined with Coomassie brilliant blue G250. In a separate study, chitosan films were placed into the test tubes with buffer solution and 10(7) U/L lysozyme. The degradation rate was calculated. Osteoblasts derived from fetal rat calvarial were cultured on chitosan films. Cell proliferation was tested by methyl thiazolyl tetrazolium (MTT) assay. The relative growth rate was calculated and the cytotoxicity was graded.
Results: The best processing condition was 1% acetic acid, chitosan concentration of 2 mg/mL, 6% sodium tripolyphosphate (STPP) concentration, and cross-linking time of one hour. The resulting chitosan film released 33.13% of bovine serum albumin (BSA) within 8 d, 36.73% of BSA within four weeks and the cytotoxicity grade was 0 or 1.
Conclusion: This chitosan film possesses good sustained release property, and a good degradation rate.
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