Backbone 1H, 13C, and 15N NMR assignments for the Cyanothece 51142 protein cce_0567: a protein associated with nitrogen fixation in the DUF683 family.

Biomol NMR Assign

Biological Sciences Division, Pacific Northwest National Laboratory, Mail-Stop K8-98, P.O. Box 999, Richland, WA 99352, USA.

Published: June 2008

AI Article Synopsis

  • Cyanothece 51142 has a 78-residue protein called cce_0567, which is linked to nitrogen fixation and belongs to the DUF683 protein family.
  • The study describes the resonance assignments for the main chain and 13C(beta) side chain of this protein.
  • The protein is characterized as a homo-tetramer with an approximate molecular weight of 40 kDa.

Article Abstract

Cyanothece 51142 contains a 78-residue protein, cce_0567, that falls into the DUF683 family of proteins associated with nitrogen fixation. Here we report the assignment of most of the main chain and 13C(beta) side chain resonances of the approximately 40 kDa homo-tetramer.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12104-007-9075-3DOI Listing

Publication Analysis

Top Keywords

cyanothece 51142
8
protein cce_0567
8
associated nitrogen
8
nitrogen fixation
8
duf683 family
8
backbone 13c
4
13c 15n
4
15n nmr
4
nmr assignments
4
assignments cyanothece
4

Similar Publications

A unicellular cyanobacterium relies on sodium energetics to fix N.

Nat Commun

November 2024

Shenzhen Public Platform for Screening and Application of Marine Microbial Resources, Tsinghua Shenzhen International Graduate School, Shenzhen, Guangdong Province, PR China.

Article Synopsis
  • Diazotrophic cyanobacteria typically fix nitrogen gas, but are rarely found in coastal areas with limited nitrogen, leading to an ecological mystery.
  • Research shows that a specific coastal cyanobacterium, Cyanothece sp. ATCC 51142 (now called Crocosphaera subtropica), requires sodium ions for nitrogen fixation and is inhibited at low sodium chloride concentrations.
  • Despite producing nitrogenase enzymes when sodium is absent, the organism fails to fix nitrogen or grow due to low ATP supply, indicating that sodium plays a crucial role in its energy metabolism for N fixation.
View Article and Find Full Text PDF

Under standard laboratory conditions, PCC 7942 lacks EcaA, a periplasmic carbonic anhydrase (CA). In this study, a transformant was created that expressed the homologous EcaA from sp. ATCC 51142.

View Article and Find Full Text PDF

The discovery of nitrogen fixation in unicellular cyanobacteria provided the first clues for the existence of a circadian clock in prokaryotes. However, recalcitrance to genetic manipulation barred their use as model systems for deciphering the clock function. Here, we explore the circadian clock in the now genetically amenable Cyanothece 51142, a unicellular, nitrogen-fixing cyanobacterium.

View Article and Find Full Text PDF

A novel salt- and organic solvent-tolerant phosphite dehydrogenase from sp. ATCC 51142.

Front Bioeng Biotechnol

August 2023

Unit of Biotechnology, Division of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, Japan.

Phosphite dehydrogenase (PtxD) is a promising enzyme for NAD(P)H regeneration. To expand the usability of PtxD, we cloned, expressed, and analyzed PtxD from the marine cyanobacterium sp. ATCC 51142 (Ct-PtxD).

View Article and Find Full Text PDF

Fluorescence Analysis of Biocide Efficiency in Antifouling Coatings against Cyanobacteria.

Int J Mol Sci

March 2023

Faculty of Geology, Geophysics and Environmental Protection, AGH-University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, Poland.

This study focused on the antifouling effect of copper oxide (CuO)- and zineb-based coatings against sp. by analysing photosynthetic activity using chlorophyll fluorescence. The photoautotrophically grown cyanobacterium was exposed to toxic coatings over a short-term period of 32 h.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!