The backbone and side chain resonance assignments of an engineered intein based on Mycobacterium tuberculosis RecA have been determined based on triple-resonance experiments with the uniformly [(13)C,(15)N]-labeled protein.
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http://dx.doi.org/10.1007/s12104-008-9098-4 | DOI Listing |
BMC Bioinformatics
January 2025
Solu Healthcare Oy, Kalevankatu 31 A 13, 00100, Helsinki, Finland.
Background: Genomic surveillance is extensively used for tracking public health outbreaks and healthcare-associated pathogens. Despite advancements in bioinformatics pipelines, there are still significant challenges in terms of infrastructure, expertise, and security when it comes to continuous surveillance. The existing pipelines often require the user to set up and manage their own infrastructure and are not designed for continuous surveillance that demands integration of new and regularly generated sequencing data with previous analyses.
View Article and Find Full Text PDFSci Rep
January 2025
Department of Applied Computer Science and Modelling Department, Faculty of Metals Engineering and Industrial Computer Science, AGH University of Krakow, Czarnowiejska 66, 30-054, Krakow, Poland.
CT images of castings made of ductile iron were analyzed in the paper. On these images, objects can be identified that can be considered as graphite precipitates or indicate the presence of a defect in the casting. Research conducted in this area is described, based on experimental data that allows to determine whether the indicated components present in the casting are graphite precipitation.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
January 2025
Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, 160 00 Prague, Czech Republic. Electronic address:
Model P-chirogenic phosphonates derived from isopinocampheol, offering an excellent experimental system for studying chirality on the phosphorus chiral center, were studied using a set of chiroptical methods including ECD, VCD and ROA. Thanks to their rigidity, limiting the number of possible conformers, we successfully correlated the experimental UV-vis/ECD, IR/VCD and Raman/ROA results with DFT calculations. This allowed us to confidently assign the absolute configuration of our models, and our assignment is consistent with X-ray diffraction (XRD) data.
View Article and Find Full Text PDFDev Psychol
January 2025
School of Communication, Ohio State University.
We investigated the impact of parents' open-ended questions during collaborative science activities. Specifically, we randomly assigned 116 parents (69.8% mothers; 89.
View Article and Find Full Text PDFThe objective of this study was to investigate the effect of the () SS18-50 (an isolate with favorable probiotic properties following space traveling) on dextran sulfate sodium (DSS)-induced colitis in mice. Male ICR mice were randomly assigned to one of six groups: a control group, a model group, and four intervention groups comprising the isolate (SS18-50-L and SS18-50-H) and the wild type (GS18-L and GS18-H) strains. The model group and the intervention groups were administered a 3.
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