AI Article Synopsis
- Researchers created isotopically labeled forms of a protein called MsrB1 from mice by substituting its key amino acid selenocysteine with cysteine.
- The study focuses on the NMR (Nuclear Magnetic Resonance) assignment for the reduced version of this protein, which is important for understanding its structure and function.
- The isotopes 15N and 13C were used to enhance the sensitivity and resolution of the NMR analysis.
Article Abstract
Isotopically labeled, 15N and 15N/13C forms of recombinant methionine-r-sulfoxide reductase 1 (MsrB1, SelR) from Mus musculus were produced, in which catalytic selenocysteine was replaced with cysteine. We report here the 1H, 13C and 15N NMR assignment of the reduced form of this mammalian protein.
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| http://dx.doi.org/10.1007/s12104-007-9039-7 | DOI Listing |
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