A radiorespirometric method for measuring mineralization of [14C]-compounds in a 96-well microplate format.

J Microbiol Methods

Geological Survey of Denmark and Greenland (GEUS), Department of Geochemistry, Øster Voldgade 10, DK-1350 Copenhagen K, Denmark.

Published: October 2009

A radiorespirometric method was developed for quantifying mineralization of [14C]-labeled substrates in deep-well microplates. Ninety-six 14CO2-traps were placed on an adhesive microplate sealing tape which made it possible to trap 14CO2 simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.

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http://dx.doi.org/10.1016/j.mimet.2009.07.018DOI Listing

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