A radiorespirometric method was developed for quantifying mineralization of [14C]-labeled substrates in deep-well microplates. Ninety-six 14CO2-traps were placed on an adhesive microplate sealing tape which made it possible to trap 14CO2 simultaneously from all wells. Trapped radioactivity was quantified by digital autoradiography.
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http://dx.doi.org/10.1016/j.mimet.2009.07.018 | DOI Listing |
The present study evaluated biochemical as well as biophysical mechanisms behind the synergistic effects of curcumin and resveratrol during prostate carcinogenesis. The rats were segregated into five groups that included normal control, 3,2'-dimethyl-4-aminobiphenyl (DMAB)treated, DMAB + curcumin treated, DMAB + resveratrol-treated and DMAB + curcumin + resveratrol-treated. The DMAB treatment resulted in a significant increase in the levels of lipid peroxidation (LPO) in DMAB treated rats.
View Article and Find Full Text PDFJ Pharmacol Toxicol Methods
April 2016
Antimicrobial Agents Unit, Laboratory for Phytobiochemistry and Medicinal Plants Study, Faculty of Science, University of Yaoundé 1, P.O. 812 Yaoundé, Cameroon.
Buruli ulcer (BU) caused by Mycobacterium ulcerans is the third most common chronic mycobacterial infection in humans. Approximately 5000 cases are reported annually from at least 33 countries around the globe, especially in rural African communities. Even though anti-mycobacterial therapy is often effective for early nodular or ulcerative lesions, surgery is sometimes employed for aiding wound healing and correction of deformities.
View Article and Find Full Text PDFPest Manag Sci
February 2015
Department of Geochemistry, Geological Survey of Denmark and Greenland, Copenhagen, Denmark.
Background: The herbicide 4-chloro-2-methylphenoxyacetic acid (MCPA) is found frequently in Danish groundwater in concentrations exceeding the EU threshold limit of 0.1 µg L(-1) . Groundwater is used for drinking water, and one potential remediation strategy is bioaugmentation using inoculation of sand filters at affected waterworks with degrader bacteria.
View Article and Find Full Text PDFPLoS One
January 2015
Department of Biophysics, Panjab University, Chandigarh, India.
Background: The combination approach is the future of the war against cancer and the present study evaluated molecular mechanics behind the synergistic effects of curcumin and resveratrol during lung carcinogenesis.
Methods: The mice were segregated into five groups which included normal control, Benzo[a]pyrene[BP] treated, BP+curcumin treated, BP+resveratrol treated and BP+curcumin+resveratrol treated.
Results: The morphological analyses of tumor nodules confirmed lung carcinogenesis in mice after 22 weeks of single intra-peritoneal[i.
Mineralization of organic chemicals in soil is typically studied using large homogenized samples, but little is known about the small-scale spatial distribution of mineralization potential. We studied centimeter-scale spatial distribution of 2-methyl-4-chlorophenoxyacetic acid (MCPA) mineralization activity at different depths (8-115 cm) in a Danish agricultural soil profile using a 96-well microplate C-radiorespirometric method for small-volume samples. The heterotrophic microbial population and specific MCPA degraders decreased 10- to 100-fold from the plow layer to a depth of 115 cm.
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