The micropipette aspiration technique was improved by selecting the uniform cells in round shape (UCR) from a number of adhered cells on the testing surface so as to measure the adhesion forces during the initial adhesion stage. Silk fibroin film and silk fibroin/fucoidan film were used as testing substrates. The testing results revealed that the modified micropipette method was more rapid for testing. The tendency of cell adhesion force on different materials surface under different conditions remained unchanged. The testing results showed good reproducibility. It was easy to distinguish small differences in the adhesiveness of endothelial cell on the silk fibroin based materials, thus exhibiting the high sensitivity of the improved method.
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Article Synopsis
- The micro-pipette aspiration technique is used to study the physical properties of fluids and biological materials, focusing on how fluids behave under varying suction pressures.
- A new mathematical model based on Cahn-Hilliard-Navier-Stokes equations is introduced to analyze the dynamics of cell aggregates in the aspiration-retraction process, taking into account different wetting conditions.
- The study offers insights into the implications of model assumptions on physical parameters and provides a new understanding of the action-reaction relationship between aspiration and retraction of cell aggregates.
Synapsin and α-synuclein represent a growing list of condensate-forming proteins where the material states of condensates are directly linked to cellular functions (e.g., neurotransmission) and pathology (e.
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