[Identification of the interactions between JTV1 and NLS-RAR alpha in vivo and in vitro].

Objective: To identify the interactions between JTV1 and NLS-RAR alpha by Yeast two-hybrid and co-immunoprecipitation.

Methods: The plasmids of bait-protein and JTV1 protein were cotransformed into yeast AH109 to investigate their interactions in vivo. Tagged fusion protein eukaryotic expression vectors were constructed and then cotransfected into human embryo kidney 293 cells. Co-immunoprecipitation was used to investigate the interactions between NLS-RAR alpha and JTV1 in vitro.

Results: Blue clones were found in QDO/X-alpha-gal plates. Eukaryotic expression vectors were co-transfected into HEK 293 cells. The HA-NLS-RAR alpha protein was immunoprecipitated by anti-HA polyclonal antibody. Myc-JTV1 protein was detected by western blotting with anti-Myc monoclonal antibody from the immunoprecipitared complex.

Conclusion: The interactions between NLS-RAR alpha and JTV1 are identified by Yeast two-hybrid and co-immunoprecipitation.