[Oxygen deficiency increases invasive activity and resistance of Yersinia pseudotuberculosis to heat stress].

Aim: To study effects of oxygen availability and presence of glucose in growth medium on adhesive and invasive properties of Yersinia pseudotuberculosis as well as its resistance to heat stress during sharp rise of temperature from 8 degrees C to 37 degrees C.

Materials And Methods: Yersinia pseudotuberculosis was grown on nutrient broth with or without glucose at 8 degrees C and two regimen of aeration--during intensive stirring (180 rpm) and without it. Adhesive and invasive activities were studied on the model of HeLa human cell line. Effects of temperature stress on the bacterial growth were assessed from growth curves plotted on the basis of quantity of colony-forming cells. Morphology of bacterial cells was studied by electron microscopy.

Results: It was shown that cultivation of Y. pseudotuberculosis at 8 degrees C and low aeration increases its adhesive and invasive activity as well resistance to heat stress. Adding of glucose to growth medium decreases invasiveness of Y. pseudotuberculosis irrespective to aeration regimen.

Conclusion: Oxygen deficiency during low temperature of growth promotes increasing of pathogenic potential of Y. pseudotuberculosis. Obtained data are useful for solving practical problems associated with development of prevention measures for pseudotuberculosis as well with food processing and storage.