Modification of comet analysis is proposed for obtaining permanent preparations by DNA staining with silver compounds. The sensitivity of staining is similar to that observed at the treatment by ethidium bromide and other fluorochromes. The advantages of the method are stability of slides and possibility of their reinvestigation by light microscopy. The method does not need expensive fluorescent microscope and lacks contacting with carcinogenic compounds and UV light irradiation.
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Chemistry
January 2025
Fujian Normal University, School of Chemistry and Materials, No.8 Shangsan Road, ., Fuzhou City, CHINA.
The advancement of high-value CH4 purification technology within the natural gas industry is paramount for industrial processes. Herein, we constructed ZJNU-402, a new porous material characterized by permanent porosity, as an effective adsorbent for separating C3H8/CH4 and C2H6/CH4 mixtures. The findings reveal an outstanding C3H8 adsorption capacity of 68 cm3 g-1 and a moderate C2H6 adsorption rate of 42 cm3 g-1, with a notably lower CH4 adsorption rate of 11 cm3 g-1.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Fraunhofer Institute IZI (Leipzig), Department Rostock, Schillingallee 68, 18057 Rostock, Germany.
Recent research has concentrated on the development of suitable in vitro cell models for the early identification of hepatotoxicity during drug development in order to reduce the number of animal models and to obtain a better predictability for hepatotoxic reactions in humans. The aim of the presented study was to identify translational biomarkers for acute liver injury in human patients that can serve as biomarkers for hepatocellular injury in vivo and in vitro in simple cell models. Therefore, 188 different metabolites from patients with acute-on-chronic liver failure before and after liver transplantation were analyzed with mass spectrometry.
View Article and Find Full Text PDFHealth Aff (Millwood)
January 2025
Julie Maslowsky, University of Michigan, Ann Arbor, Michigan.
Young adults' access to contraception is shifting after the June 2022 United States Supreme Court decision. This concurrent mixed-methods study measured young adults' use of and perceptions about tubal sterilization and vasectomy after the leaked opinion in May 2022. Using national-level medical claims data from IQVIA, we conducted difference-in-differences analyses of tubal sterilizations and vasectomies by age and state policy; using open-text survey responses from national MyVoice surveys in 2022 and 2023, we thematically analyzed young adults' perspectives.
View Article and Find Full Text PDFArtificial heterostructures are often realized by stacking different materials to present new emerging properties that are not exhibited by their individual constituents. In this work, non-layered two-dimensional α-MnSe nanosheets were transferred onto LaMnO (LMO) films to obtain a multifunctional heterostructure. The high crystal quality of the MnSe/LMO heterostructure was revealed by X-ray diffraction, Raman spectroscopy, and scanning electron microscopy measurements.
View Article and Find Full Text PDFBMC Geriatr
January 2025
Department of Orthopaedic Surgery, The Third Hospital of Hebei Medical University, No.139, ZiQiang Lu, Shijiazhuang, Hebei, 050051, PR China.
Background: A scarcity of data exists concerning atrial fibrillation (AF) during the perioperative stage of non-cardiothoracic surgery, particularly orthopedic surgery. In addition, given the frequency and significant impact of AF in the perioperative period, therefore our aim was to identify prognosis and predictors of elderly hip fracture patients with perioperative AF.
Methods: An examination of hip fracture patients at the Third Hospital of Hebei Medical University, who had been hospitalized from January 2018 to October 2020 in succession, was conducted retrospectively.
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