The hydrophobic and hydrophilic properties of the surface of poly-ether sulfone (PES) films were controlled by an atmospheric pressure plasma (AP) treatment using reactive gases (Ar/H(2) and Ar/O(2)). The surface properties of the Ar/H(2) and Ar/O(2) in series AP-treated PES films showed higher surface roughness (approximately 120%), surface energy (approximately 30%) and hydrophilic properties (oxygen content approximately 10%) than the Ar/O(2) AP-treated PES film. The protein staining results confirmed that an activated region on the patterned PES film with high selectivity and sensitivity was well-defined and formed. This method is suitable for fabricating flexible protein adhesive chips with uniform biomolecular adhesive properties.
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