Pandemic influenza A viruses that emerge from animal reservoirs are inevitable. Therefore, rapid genomic analysis and creation of vaccines are vital. We developed a multisegment reverse transcription-PCR (M-RTPCR) approach that simultaneously amplifies eight genomic RNA segments, irrespective of virus subtype. M-RTPCR amplicons can be used for high-throughput sequencing and/or cloned into modified reverse-genetics plasmids via regions of sequence identity. We used these procedures to rescue a contemporary H3N2 virus and a swine origin H1N1 virus directly from human swab specimens. Together, M-RTPCR and the modified reverse-genetics plasmids that we designed streamline the creation of vaccine seed stocks (9 to 12 days).
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| http://dx.doi.org/10.1128/JVI.01109-09 | DOI Listing |
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- The bacterial pathogen, significant in both animals and humans, is linked to a variety of infections, highlighting the need to understand its genomic and metabolic features for effective control.
- Whole-genome sequencing and antimicrobial susceptibility testing were utilized to analyze 83 genomes from multiple animal hosts, revealing that the strains are not specific to hosts or body sites, indicating a broader infection potential.
- The study found conserved virulence genes and metabolic versatility in the pathogen, which may enhance its survival and competitiveness, raising concerns about its zoonotic potential and health impacts on humans and animals.
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