This study demonstrates the application of Wavelength-Scanned Cavity Ring-Down Spectroscopy (WS-CRDS) technology which is used to measure the stable isotopic composition of water. This isotopic water analyzer incorporates an evaporator system that allows liquid water as well as water vapor to be measured with high precision. The analyzer can measure H2(18)O, H2(16)O and HD(16)O content of the water sample simultaneously. The results of a laboratory test and two field trials with this analyzer are described. The results of these trials show that the isotopic water analyzer gives precise, accurate measurements with little or no instrument drift for the two most common isotopologues of water. In the laboratory the analyzer has a precision of 0.5 per mil for deltaD and 0.1 per mil for delta(18)O which is similar to the precision obtained by laboratory-based isotope ratio mass spectrometers. In the field, when measuring vapor samples, the analyzer has a precision of 1.0 per mil for deltaD and 0.2 per mil for delta(18)O. These results demonstrate that the isotopic water analyzer is a powerful tool that is appropriate for use in a wide range of applications and environments.
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http://dx.doi.org/10.1002/rcm.4100 | DOI Listing |
Plant Cell Environ
January 2025
Civil, Environmental, and Mining Engineering, University of Western Australia, Perth, Western Australia, Australia.
Understanding and predicting plant water dynamics during and after water stress is increasingly important but challenging because the high-dimensional nature of the soil-plant-atmosphere system makes it difficult to identify mechanisms and constrain behaviour. Datasets that capture hydrological, physiological and meteorological variation during changing water availability are relatively rare but offer a potentially valuable resource to constrain plant water dynamics. This study reports on a drydown and re-wetting experiment of potted Populus trichocarpa, which intensively characterised plant water fluxes, water status and water sources.
View Article and Find Full Text PDFEnviron Sci Process Impacts
January 2025
Massachusetts Institute of Technology, Department of Civil and Environmental Engineering, Parsons Laboratory, 15 Vassar Street, Cambridge, Massachusetts 02139, USA.
The high salinity and organic content in oil and gas wastewaters can cause ion suppression during liquid chromatography mass spectrometry (LC/MS) analysis, diminishing the sensitivity and accuracy of measurements in available methods. This suppression is severe for low molecular weight organic compounds such as ethanolamines (, monoethanolamine (MEA), diethanolamine (DEA), triethanolamine (TEA), -methyldiethanolamine (MDEA), and ,-ethyldiethanolamine (EDEA)). Here, we deployed solid phase extraction (SPE), mixed-mode LC, triple quadrupole MS with positive electrospray ionization (ESI), and a suite of stable isotope standards (, one per target compound) to correct for ion suppression by salts and organic matter, SPE losses, and instrument variability.
View Article and Find Full Text PDFEcology
January 2025
Center for Ecosystem Science and Society, Department of Biology, Northern Arizona University, Flagstaff, Arizona, USA.
Fungi play a crucial role in aquatic leaf litter decomposition. Aquatic fungi have long been thought to spend the majority of their lives in the water. Here, we explore the possibility of an amphibious life cycle, where phyllosphere fungi spend part of their life cycle in aquatic systems.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
January 2025
Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550.
In soils, the first rain after a prolonged dry period represents a major pulse event impacting soil microbial community function, yet we lack a full understanding of the genomic traits associated with the microbial response to rewetting. Genomic traits such as codon usage bias and genome size have been linked to bacterial growth in soils-however, often through measurements in culture. Here, we used metagenome-assembled genomes (MAGs) with O-water stable isotope probing and metatranscriptomics to track genomic traits associated with growth and transcription of soil microorganisms over one week following rewetting of a grassland soil.
View Article and Find Full Text PDFFood web architecture and trophic interactions between organisms can be studied using ratios of naturally occurring stable isotopes of carbon (C/C) and nitrogen (N/N). Most studies, however, focused on free-living organisms, but recently, there has been growing interest in understanding trophic interactions of parasites. The crustacean ectoparasite is a well-studied parasite of freshwater teleost fish, which has low host specificity and a cosmopolitan distribution.
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