We describe a device for automated fragmentation of genomic DNA by hydrodynamic shearing using a filter screen with uniform pores. Human genomic DNA can be fragmented reproducibly to a range of 2000-12,000 bp by using various fluid flow rates and screens with 0.5-10-microm pores. The utilization of disposable screens eliminates sample cross-contamination and the tendency of device clogging, commonly encountered in single-orifice shearing devices.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2880395 | PMC |
| http://dx.doi.org/10.2144/000113123 | DOI Listing |
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