Identification of two lipid binding proteins from liver of Gallus domesticus.

1. Two low molecular weight (approximately 14,000 Da) proteins exhibiting lipid binding activity were purified from liver cytosol and identified as non-specific lipid binding protein (ns-LTP) and fatty acid binding protein (L-FABP). 2. Ligand binding assays indicated that ns-LTP exhibited greater binding activity for cholesterol and little binding of fatty acids. Conversely, L-FABP had higher relative binding activity for fatty acids but did not bind cholesterol. 3. Amino acid composition and pI data supported the identification of the chicken liver lipid binding proteins as L-FABP and ns-LTP. 4. Polyclonal antisera was prepared against each of the liver lipid binding proteins and monospecificity verified using Western blot analysis.