In this unit, we describe a set of improvements we have made to the standard Illumina Genome Analyzer protocols to make the sequencing process more reliable in a high-throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3849550 | PMC |
| http://dx.doi.org/10.1002/0471142905.hg1802s62 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Precision medicine for patients with salivary gland neoplasms: Determining the feasibility of implementing a next-generation sequencing-based RNA assay in a hospital laboratory.
Cytojournal
November 2024
Department of Pathology and Genomic Medicine, Houston Methodist, Houston, Texas, USA.
Objective: Diagnosing neoplasms of the salivary gland is challenging, as morphologic features of these tumors are complex, and well-defined diagnostic categories have overlapping features. Many salivary gland neoplasms are associated with recurrent genetic alterations. The utilization of RNA-based targeted next-generation sequencing (NGS) panels for the detection of cancer-driving translocations and mutations is emerging in the clinical laboratory.
View Article and Find Full Text PDFNewly Isolated LAMA1607 for Enhanced Biological Phosphorus Removal: A Genomic and Functional Characterization.
Front Biosci (Elite Ed)
December 2024
Polytechnic School, University of Vale do Itajaí (Univali), Itajaí, SC 88302-202, Brazil.
Background: Enhanced biological phosphorus removal (EBPR) systems utilize phosphorus-accumulating organisms (PAOs) to remove phosphorus from wastewater since excessive phosphorus in water bodies can lead to eutrophication. This study aimed to characterize a newly isolated PAO strain for its potential application in EBPR systems and to screen for additional biotechnological potential. Here, sequencing allowed for genomic analysis, identifying the genes and molecules involved, and exploring other potentials.
View Article and Find Full Text PDFGenotype frequency analysis of rs2025804 genetic variant in Iranian population.
J Diabetes Metab Disord
June 2025
Personalized Medicine Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, No. 10- Jalal -e-Ale-Ahmad Street, Chamran Highway, Tehran, 1411713119 Iran.
Objectives: The gene is a key focus in obesity research, with studies linking its polymorphisms to various diseases like polycystic ovarian syndrome and energy intake disorders. This study aims to investigate the prevalence of the rs2025804 variant within LEPR and its distribution among healthy individuals across diverse ethnic groups in Iran.
Methods: The frequency of the rs2025804 genotype in the gene was analyzed in 1142 healthy adults representing different ethnicities in Iran.
Tracking SARS-CoV-2 variants in wastewater in San Pedro de la Paz, Chile.
J Water Health
December 2024
Centro de Vigilancia de Aguas Residuales, Centinela Biobío, Facultad de Medicina, Universidad Católica de la Santísima Concepción, Concepción, Chile; Laboratorio de Investigación en Ciencias Biomédicas, Departamento de Ciencias Básicas y Morfología, Facultad de Medicina, Universidad Católica de la Santísima Concepción, Concepción, Chile E-mail:
Studies have shown the presence of SARS-CoV-2 in the stool of both symptomatic and asymptomatic COVID-19 patients, enabling wastewater-based surveillance (WBS) to complement clinical monitoring. The emergence of variants can enhance viral transmissibility, highlighting the need for ongoing surveillance to detect and control infectious diseases. This study aimed to detect SARS-CoV-2 variants in wastewater from a treatment plant in San Pedro de la Paz, Chile, between January and November 2021.
View Article and Find Full Text PDFComplete mitochondrial genome sequencing and phylogenetic analysis of Phellinus igniarius.
Sci Rep
December 2024
College of Life sciences, Shandong First Medical University and Shandong Academy of Medical Sciences, Tai'an, 271016, China.
The mitochondrial whole genome of Phellinus igniarius was sequenced with the objective of examining the evolutionary relationships amongst related species. The entire mitochondrial genome was assembled using Illumina sequencing technology. The structural annotation and bioinformatics analysis were performed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!