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Decontamination of DNA sequences from a Streptomyces genome for optimal genome mining.
Braz J Microbiol
January 2025
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, SP, 05508-900, Brazil.
Despite meticulous precautions, contamination of genomic DNA samples is not uncommon, which can significantly compromise the analysis of microorganisms' whole-genome sequencing data, thus affecting all subsequent analyses. Thanks to advancements in software and bioinformatics techniques, it is now possible to address this issue and prevent the loss of the entire dataset obtained in a contaminated whole-genome sequencing, where the DNA of another bacterium is present. In this study, it was observed that the sequencing reads from Streptomyces sp.
View Article and Find Full Text PDFAmmonia and beyond - biomarkers of hepatic encephalopathy.
Metab Brain Dis
January 2025
Fundación de Investigación Hospital Clínico Universitario de Valencia-INCLIVA, Valencia, 46010, Spain.
Ammonia is a product of amino acid metabolism that accumulates in the blood of patients with liver cirrhosis, leading to neurotoxic effects and hepatic encephalopathy (HE). HE manifestations can range from mild, subclinical disturbances in cognition, or minimal HE (mHE) to gross disorientation and coma, a condition referred to as overt HE. Many blood-based biomarkers reflecting these neurotoxic effects of ammonia and liver disease can be measured in the blood allowing the development of new biomarkers to diagnose cirrhosis patients at risk of developing HE.
View Article and Find Full Text PDFReturn to work of major trauma survivors from a private level 1 trauma centre in South Africa.
Eur J Trauma Emerg Surg
January 2025
Division of Physiotherapy, Department of Health and Rehabilitation Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.
Purpose: Trauma is known as a leading cause of mortality and injury related disability globally. In South Africa (SA) the socioeconomic burden of trauma is magnified as the working age is most affected. The aim of this study was to describe the proportion of major trauma survivors who returned to work (RTW) during a 6-month period post hospital discharge and to identify the factors associated with the RTW outcome.
View Article and Find Full Text PDFCivil Disobedience in Democratic Education.
Integr Psychol Behav Sci
January 2025
University of Delaware, Newark, DE, USA.
The study's goal was to examine the tension between democratic school governance, requiring its participants to obey school rules, even though they might disagree with those rules, and personal responsibility, requiring the participants to act morally, in accordance with their conscience and their sense of what is right and makes sense for them, regardless of the democratic nature of the imposed school rules. This examination was based on three sources: 1) three Open Symposia with American and Russian democratic educationalists, 2) my review of the existing literature on civil disobedience and democratic education, and 3) my empirical study based on the interviews of participants of an American private democratic school, known as The Circle School, regarding their instances of civil disobedience. The three Open Symposia allowed me to develop a working definition of civil disobedience as a particular principled disobedience.
View Article and Find Full Text PDFEvaluating the value of 3D-printed bone models with fracture fragments connected by flexible rods for training and preoperative planning.
3D Print Med
January 2025
AO Innovation Translation Center (AO ITC), AO Foundation, Davos, Switzerland.
Background: The emergence of 3D printing has revolutionized medical training and preoperative planning. However, existing models have limitations, prompting the development of newly designed flexible 3D-printed bone fracture models.
Methods: The designed flexible 3D-printed bone fracture models were evaluated by 133 trauma surgeons with different levels of experience for perceived value as educational tool or as preoperative planning tool.
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