Frankly, I was surprised to receive an invitation to write a prefatory chapter for the Annual Review of Microbiology. I have read several such chapters written by outstanding researchers, many of whom I know and admire. I did not think I belonged to such a preeminent group. In my view, my contributions to the physiology and biochemistry of anaerobic thermophilic bacteria and, more lately, to anaerobic fungi are modest compared to the contribution made by other authors of prefatory chapters. I am honored to write about my life and my work, and I hope that those who read this chapter will sense how exciting and rewarding they have been.
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http://dx.doi.org/10.1146/annurev.micro.091208.073617 | DOI Listing |
Trends Biotechnol
January 2025
School of Environment and Energy Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea; Research Center for Innovative Energy and Carbon Optimized Synthesis for Chemicals, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea. Electronic address:
(Homo)acetogens, including Clostridium spp., represent an enigma in metabolic flexibility and diversity. Eubacterium callanderi KIST612 is an acetogen that produces n-butyrate with carbon monoxide (CO) as the carbon and energy source; however, the production route is unknown.
View Article and Find Full Text PDFMolecules
November 2024
Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
The use of mixed cultures in gas fermentations could reduce operating costs in the production of liquid chemicals such as alcohols or carboxylic acids. However, directing reducing equivalents towards the desired products presents the challenge of co-existing competing pathways. In this study, two trickle bed reactors were operated at acetogenic and chain elongating conditions to explore the fate of electron equivalents (ethanol, H, and CO) and test pH oscillations as a strategy to target chain-elongated products.
View Article and Find Full Text PDFBioresour Technol
December 2024
Centre for Sustainable Future Technologies, Fondazione Istituto Italiano di Tecnologia, Via Livorno 60, Turin 10144, Italy. Electronic address:
Natl Sci Rev
November 2024
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Stress Biology and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
Candidate bacterial phylum CSP1-3 has not been cultivated and is poorly understood. Here, we analyzed 112 CSP1-3 metagenome-assembled genomes and showed they are likely facultative anaerobes, with 3 of 5 families encoding autotrophy through the reductive glycine pathway (RGP), Wood-Ljungdahl pathway (WLP) or Calvin-Benson-Bassham (CBB), with hydrogen or sulfide as electron donors. Chemoautotrophic enrichments from hot spring sediments and fluorescence hybridization revealed enrichment of six CSP1-3 genera, and both transcribed genes and DNA-stable isotope probing were consistent with proposed chemoautotrophic metabolisms.
View Article and Find Full Text PDFLife (Basel)
October 2024
Key Laboratory of Dark Matter and Space Astronomy, Purple Mountain Observatory, Chinese Academy of Sciences, Nanjing 210023, China.
According to panspermia, life on Earth may have originated from life forms transported through space from elsewhere. These life forms could have passed through molecular clouds, where the process of methanogenesis could have provided enough energy to sustain living organisms. In this study, we calculate the Gibbs free energy released from synthesizing hydrocarbons for methanogenic (acetogenic) life in a molecular cloud, with methane (acetic acid) as the final metabolic product.
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