Detection and quantification of neurotransmitters in dialysates.

Curr Protoc Neurosci

NIH, NIDA Intramural Research Program, Baltimore, Maryland, USA.

Published: July 2009

Sensitive analytical methods are needed for the separation and quantification of neurotransmitters obtained in microdialysate studies. This unit describes methods that permit quantification of nanomolar concentrations of monoamines and their metabolites (high-pressure liquid chromatography electrochemical detection), acetylcholine (HPLC-coupled to an enzyme reactor), and amino acids (HPLC-fluorescence detection; capillary electrophoresis with laser-induced fluorescence detection).

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2950641PMC
http://dx.doi.org/10.1002/0471142301.ns0704s48DOI Listing

Publication Analysis

Top Keywords

quantification neurotransmitters
8
detection
4
detection quantification
4
neurotransmitters dialysates
4
dialysates sensitive
4
sensitive analytical
4
analytical methods
4
methods needed
4
needed separation
4
separation quantification
4

Similar Publications

Since the early discovery of QRFP43, intensive research has been primarily focused on its role in the modulation of food intake. As is widely recognised, the regulation of the body's energy status is a highly complex process involving numerous systems, hormones and neurotransmitters. Among the most important regulators of energy status, alongside the satiety and hunger centre located in the hypothalamus, is the HPT axis, which directly and indirectly affects the regulation of metabolism in all cells of the body.

View Article and Find Full Text PDF

Gamma-aminobutyric acid (GABA) has been attributed to health-promoting properties and has received attention from the food industry as an attractive bioactive compound for the development of functional foods. Some lactic acid bacteria (LAB) produce GABA through a glutamate decarboxylase encoded by B and a glutamate/GABA antiporter encoded by C. In this study, we develop a molecular screening method based on a polymerase chain reaction able to detect those genes in different LAB species through the use of novel multispecies primers.

View Article and Find Full Text PDF

In recent times, a truly exquisite pharmaceutical marvel has graced the world of medicine, known as Safinamide (SAF). This opulent creation has been specifically tailored to cater to the needs of individuals afflicted with Parkinson's disease (PD), an esteemed neurological condition renowned for its regal ability to impede motor skills, coordination, and equilibrium. It is highly improbable that degradation products of pharmaceutical components would significantly compromise efficiency and safety of a drug during its shelf life.

View Article and Find Full Text PDF

A new selective and sensitive high-performance liquid chromatography (HPLC) method was developed for the quantification of potential impurities in fluoxetine hydrochloride. Chromatographic separation was achieved on an end-capped octadecylsilyl silica gel (Gemini-C18 150 mm × 4.6 mm, 3.

View Article and Find Full Text PDF

Babassu (Atallea sp.), a native palm tree from South America's Amazon produces bio-oil and biochar with significant potential for industrial applications. Babassu oil as a bio-based plasticizer is reported here for the first time to replace petrochemical alternatives in the production of conductive filaments for additive manufacturing purposes.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!