AI Article Synopsis
- Most genes in humans, mice, and rats can produce multiple transcript isoforms, which complicates the study of RNA production.
- The Affymetrix Exon Array helps researchers analyze RNA levels at specific exons by utilizing separate probesets.
- The Affy Exon Tissues track provides visual insights on exon expression across different normal tissues and is available for free on the UCSC Genome Browser.
Article Abstract
Summary: Most genes in human, mouse and rat produce more than one transcript isoform. The Affymetrix Exon Array is a tool for studying the many processes that regulate RNA production, with separate probesets measuring RNA levels at known and putative exons. For insights on how exons levels vary between normal tissues, we constructed the Affy Exon Tissues track from tissue data published by Affymetrix. This track reports exon probeset intensities as log ratios relative to median values across the dataset and renders them as colored heat maps, to yield quick visual identification of exons with intensities that vary between normal tissues.
Availability: Affy Exon Tissues track is freely available under the UCSC Genome Browser (http://genome.ucsc.edu/) for human (hg18), mouse (mm8 and mm9), and rat (rn4).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2735668 | PMC |
| http://dx.doi.org/10.1093/bioinformatics/btp414 | DOI Listing |
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- The Affy Exon Tissues track provides visual insights on exon expression across different normal tissues and is available for free on the UCSC Genome Browser.
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