Osmotic adaptation in a blastomere of mouse embryo has been studied by the direct measurement of the cell volume using laser scanning microscopy microtomography followed by quantitative 3D reconstruction. Embryonic cells subjected to hypotonic shock first swelled and then returned to the initial size. At the beginning of osmotic stress, the swelling occurred by the van't Hoff equation with the water permeability coefficient (L(p)) of 0.4 micro x min(-1) atm(-1). The phase of the regulatory volume decrease was not abolished by Na+/K(+)-ATPase inhibition.
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