Motif-programmed artificial proteins with mineralization-related activity were covalently immobilized onto the surface of a hydrogel, poly(2-hydroxyethyl methacrylate) (PHEMA). We investigated the influence of assaying conditions upon the ability of three selected proteins (PS64, PS382 and PS458) to modulate calcification in vitro. A long-term assay measuring the real amount of calcium phosphate phase in the protein-modified PHEMA showed that all proteins enhanced the uptake of calcium by the hydrogel. For PS382 and PS458, this is a behaviour opposite to that displayed when the same proteins were tested in a free state by a rapid solution assay. Such difference may be attributed to a restricted mobility of the proteins due to immobilization.
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Motif-programmed artificial proteins mediated nucleation of octacalcium phosphate on titanium substrates.
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We report the creation of motif-based artificial proteins that bind to titanium surfaces and mediate the deposition of crystalline calcium phosphate. These proteins enabled in aqua surface modification of titanium materials having an intricate structure used in medical applications.
View Article and Find Full Text PDF[Applications of the motif-programmed proteins in medical area].
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Motif-programming is a method for creating artificial proteins by combining functional peptide motifs in a combinatorial manner. Motifs are often short amino acid sequences within natural proteins that are associated with particular biological functions. Motifs also can be created de novo using molecular engineering.
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Queensland Eye Institute, South Brisbane, Queensland 4101, Australia.
Motif-programmed artificial proteins with mineralization-related activity were covalently immobilized onto the surface of a hydrogel, poly(2-hydroxyethyl methacrylate) (PHEMA). We investigated the influence of assaying conditions upon the ability of three selected proteins (PS64, PS382 and PS458) to modulate calcification in vitro. A long-term assay measuring the real amount of calcium phosphate phase in the protein-modified PHEMA showed that all proteins enhanced the uptake of calcium by the hydrogel.
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Proc Natl Acad Sci U S A
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Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Koto, Tokyo 135-8550, Japan.
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Motif-programming is a method for creating artificial proteins by combining functional peptide motifs in a combinatorial manner. This method is particularly well suited for developing liaison molecules that interface between cells and inorganic materials. Here we describe our creation of artificial proteins through the programming of two motifs, a natural cell attachment motif (RGD) and an artificial Ti-binding motif (minTBP-1).
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