The increase in our understanding of D-amino acid function and distribution in mammals is in many ways a result of the initial development of sensitive enantioselective separation strategies that allow for quantification in real biological samples. This article reviews progress on the development of chiral selective separation and detection of D-amino acids including enzyme-based microbiosensors, GC/MS, HPLC/fluorescence, HPLC/MS-MS, cEKC/fluorescence, and MEKC/fluorescence. Only methods capable of analyzing D-isomers in biological matrices are given here and significant effort is made to highlight approaches that offer speed, resolution, high sensitivity, and versatility.
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Article Synopsis
- Chiral metabolomics is a growing area in neuroscience that focuses on D-amino acids as potential biomarkers for neurological diseases, utilizing advanced analytical techniques like liquid chromatography-mass spectrometry (LC-MS).
- The review highlights the importance of D-amino acids in disease pathology, particularly their influence on neurotransmission, and discusses the need for sensitive methods due to their low concentration in biological samples.
- Recent advancements in chiral derivatization and LC-MS/MS techniques have improved the detection and quantification of D-amino acids, enhancing our understanding of their role in neurological disorders and drug interactions in the brain.
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