AI Article Synopsis
- Research on fluorescent proteins is yielding new mutations that can change color when exposed to UV or blue light, with a discovery of EYFP converting to a CFP-like protein under green light.
- The photoconversion process is influenced by temperature and occurs mainly in fixed, open cell samples, potentially creating a bright, stable cyan-like fluorescent protein.
- Variation in photoconversion efficiency suggests it complicates pbFRET analysis, so samples should be discarded if photoconversion is detected to ensure accurate results.
Article Abstract
Ongoing research efforts into fluorescent proteins continuously generates new mutation variants, some of which can become photoactivated or photoconverted to a red-shifted color upon intense UV or blue light illumination. We report a built-in propensity for enhanced yellow fluorescent protein (EYFP) to undergo irreversible photoconversion into a cyan fluorescent protein (CFP)-like species upon green-light illumination. The photoconversion is thermally activated, happens mainly in fixed, nonsealed cell samples, and may result in a very bright and relatively photostable CFP-like species. The photoconversion efficiency depends on the sample diffusivity and is much increased in dehydrated, oxygenated samples. Given the large variations in conversion efficiency observed among samples as well as within a sample, photoconversion cannot be appropriately accounted for in the analysis of acceptor photobleaching fluorescence resonance energy transfer (pbFRET) images and should rather be completely avoided. Thus, samples should always be checked and discarded if photoconversion is observed.
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| http://dx.doi.org/10.1117/1.3103338 | DOI Listing |
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