AI Article Synopsis
- Two DNA-based methods were developed to analyze livestock diets by characterizing plant DNA in feces.
- The first method creates a unique pattern through electrophoresis after PCR amplification of plant DNA, while the second employs pyrosequencing to identify multiple plant species present in the diet.
- Both methods were validated using fecal samples from different feeding regimes, successfully distinguishing between concentrate and pasture diets and providing complementary data to existing chemical analysis techniques.
Article Abstract
Because of the demand for controlling livestock diets, two methods that characterize the DNA of plants present in feces were developed. After DNA extraction from fecal samples, a short fragment of the chloroplastic trnL intron was amplified by PCR using a universal primer pair for plants. The first method generates a signature that is the electrophoretic migration pattern of the PCR product. The second method consists of sequencing several hundred DNA fragments from the PCR product through pyrosequencing. These methods were validated with a blind analysis of feces from concentrate- and pasture-fed lambs. The signature method allowed differentiation of the two diets and confirmed the presence of concentrate in one of them. The pyrosequencing method allowed the identification of up to 25 taxa in a diet. These methods are complementary to the chemical methods already used. They could be applied to the control of diets and the study of food preferences.
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