AI Article Synopsis
- - A new set of vectors has been created to improve the accuracy and flexibility of targeted homologous recombination.
- - Recombinase-mediated cassette exchange (RMCE) allows for multiple modifications at any specific site, making it easier to introduce custom mutations.
- - The process is demonstrated by using RMCE to remove a specific intronic microRNA gene, showcasing its practical application.
Article Abstract
A series of vectors has been designed to enhance the versatility of targeted homologous recombination. Recombinase-mediated cassette exchange permits sequential targeting at any locus and improves flexibility in making user-defined mutations. Application of RMCE to delete an intronic microRNA gene is described.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746163 | PMC |
| http://dx.doi.org/10.1534/genetics.109.105213 | DOI Listing |
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