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Consortium of 2029 and 7247 Strains Shows In Vitro Bactericidal Effect on and, in Combination with Prebiotic, Protects Against Intestinal Barrier Dysfunction.
Antibiotics (Basel)
November 2024
Department of Biomolecular Sciences, School of Life Sciences, Chemistry and Pharmacy, Faculty of Health, Science, Social Care and Education, Kingston University London, Kingston upon Thames KT1 2EE, UK.
(CJ) is the etiological agent of the world's most common intestinal infectious food-borne disease, ranging from mild symptoms to fatal outcomes. The development of innovative synbiotics that inhibit the adhesion and reproduction of multidrug-resistant (MDR) CJ in animals and humans, thereby preserving intestinal homeostasis, is relevant. We have created a synbiotic based on the consortium of 2029 (LC2029), 7247 (LS7247), and a mannan-rich prebiotic (Actigen).
View Article and Find Full Text PDFThe Effect of Cryopreservation on T-Cell Subsets by Flow Cytometry Automated Algorithmic Analysis and Conventional Analysis.
J Clin Lab Anal
January 2025
Clinical Medical Research Center, Xinqiao Hospital, Army Medical University, Chongqing, China.
Background: Low-temperature cryopreservation is a common method for scientific research and clinical sample preservation when utilizing flow cytometry. In flow cytometry data analysis, traditional manual "gating" is susceptible to past experience and faces the challenge of manual subjective bias, time-consuming, and multidimensional data analysis. With the development of algorithms, the advantages of dimensionality reduction and clustering in result analysis are gradually becoming more prominent.
View Article and Find Full Text PDFSimplifying complexity: the novel degradation index for urban stream landscapes-case of Tekirdag.
Environ Monit Assess
December 2024
Department of Environmental Engineering, Tekirdag Namik Kemal University, Tekirdag, Turkey.
This study aims to examine the degradation process of urban stream landscapes in Tekirdağ by utilizing a newly developed degradation index (DI) that effectively defines degradation. The DI considers the vital factors that affect urban stream landscapes, making it possible to determine the level of degradation and resulting environmental quality. This index aims to provide valuable insight into the degradation of urban stream landscapes with a minimal amount of independent variables and training samples.
View Article and Find Full Text PDFNear-infrared diffuse optical characterization of human thyroid using ultrasound-guided hybrid time-domain and diffuse correlation spectroscopies.
Biomed Opt Express
December 2024
ICFO - Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels (Barcelona), Spain.
Thyroid vascularization and hemodynamics become altered in thyroid pathologies and could thus inform diagnostics, therapy planning, and follow-up. However, the current non-invasive monitoring methods available in clinics lack the necessary sensitivity and/or are impractical for large-scale deployment. As a step towards proposing a new modality, we applied the first platform, to our knowledge, designed to do simultaneous measurements of neck anatomy and thyroid microvascular hemodynamics and metabolism in a single probe placement, integrating state-of-the-art near-infrared spectroscopy techniques and clinical ultrasound.
View Article and Find Full Text PDFAn Injectable Solution for Preservation of Hematopoietic Stem and Progenitors Cells in Hypothermic Condition.
Stem Cell Rev Rep
December 2024
Etablissement Français du Sang Nouvelle Aquitaine, CS21010, Bordeaux-Cedex, 3035, France.
To ensure the preservation of functional hematopoietic stem cells (HSC) and committed progenitor cells (HPC) at + 4 °C in ex vivo expanded cord blood cell products during worldwide transportation and subsequent infusion-without the need for washing and cell concentration-we developed a conservation medium called Stabilizer of Expanded Cells (SEC), composed exclusively of injectable pharmacological products. The in vivo engraftment assay in immunodeficient mice was used to detect primitive HSCs before and after preservation at + 4 °C. In some experiments, a complex phenotype based on CD34, CD38, and CD133 expression was utilized for this purpose.
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