AI Article Synopsis
- Matriptase is a special protein found in certain cells like those in your stomach and kidneys.
- It usually hangs out on the side of the cell that faces the body’s inside (called basolateral), but how it gets there was a mystery.
- Researchers found that some specific parts of matriptase tell it where to go in the cell, and this is the first time anyone has confirmed this idea!
Article Abstract
Matriptase is a type II transmembrane serine protease. This protease is strongly expressed in simple epithelial cells such as enterocytes and kidney tubular cells in which the plasma membranes are separated into apical and basolateral domains. Although matriptase was found previously to occur exclusively on the basolateral membrane of enterocytes, the underlying mechanism of localization is unclear. In the present study, a full-length rat matriptase and a chimera consisting of the cytoplasmic and transmembrane regions of the protease and green fluorescent protein (designated as 1-86GFP) were found to localize exclusively to the basolateral membrane domain when expressed in Madin-Darby canine kidney epithelial cells. Mutagenesis analysis of 1-86GFP revealed that the matriptase cytoplasmic juxtamembrane amino acid residues (Lys45, Val47, and Arg50) play a role in mediating the localization in the cells. This study provides the first evidence that matriptase carries information for its localization in simple epithelia.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2774564 | PMC |
| http://dx.doi.org/10.1007/s10616-009-9205-0 | DOI Listing |
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