To know if thioredoxin reductase 1 (TrxR1) plays a role in antioxidant defense mechanisms against atherosclerosis, effect of TrxR1 on expression/release of monocyte chemoattractant protein (MCP-1) was investigated in activated human endothelial-like EAhy926 cells. The MCP-1 release and expression, cellular generation of reactive oxygen species (ROS), nuclear translocation and DNA-binding activity of NF-kappaB subunit p65 were assayed in cells either overexpressing recombinant TrxR1 or having their endogenous TrxR1 knocked down. It was found that overexpression of TrxR1 enhanced, while knockdown of TrxR1 reduced MCP-1 release and expression. Upregulation of MCP-1 by TrxR1 was associated with increasing generation of intracellular ROS generation, enhanced nuclear translocation and DNA-binding activity of NF-kappaB. Assay using NF-kappaB reporter revealed that TrxR1 upregulated transcriptional activity of NF-kappaB. This study suggests that TrxR1 enhances ROS generation, NF-kappaB activity and subsequent MCP-1 expression in endothelial cells, and may promote rather than prevent vascular endothelium from forming atherosclerotic plaque.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2009.06.100 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
METTL3/YTHDC1 mediates up-regulation of lncRNA OGRU in an m6A-dependent manner involving in oxidative stress and inflammation of HG-induced Müller cells.
Immunol Lett
January 2025
Department of Ophthalmology, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330006, Jiangxi Province, P.R. China; Nanchang Key Laboratory for Blindness and Visual Impairment Prevention Technology and Equipment, Nanchang 330022, Jiangxi Province, P.R. China. Electronic address:
Background: Diabetic retinopathy (DR) is a common complication of diabetes, which may cause visual disturbance and even loss of sight. Oxidative stress and inflammation are two crucial pathological factors of DR; however, their specific regulatory mechanisms in DR remain obscure.
Methods: DR models were established in streptozotocin-challenged rats and high glucose (HG)-stimulated Müller cells.
Combination of Apigenin and Melatonin with nanostructured lipid carriers as anti-inflammatory ocular treatment.
Int J Pharm
December 2024
Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, University of Barcelona, 08028 Barcelona, Spain; Institute of Nanoscience and Nanotechnology (IN(2)UB), University of Barcelona, 08028 Barcelona, Spain. Electronic address:
Ocular inflammation is a complex pathology with limited treatment options. While traditional therapies have side effects, novel approaches, such as natural compounds like Apigenin (APG) and Melatonin (MEL) offer promising solutions. APG and MEL, in combination with nanostructured lipid carriers (NLC), may provide a synergistic effect in treating ocular inflammation, potentially improving patient outcomes and reducing adverse effects.
View Article and Find Full Text PDFSIRT7 Promotes Alcohol Associated Liver Injury via Modulating Myeloid Cell CCL2 Secretion through NF-κB Signaling Pathway.
Am J Pathol
December 2024
The Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, The Key Laboratory of Model Animals and Stem Cell Biology of Hunan Province, Engineering Research Center of Reproduction and Translational Medicine of Hunan Province, and Institute of Interdisciplinary Studies, Hunan Normal University School of Pharmaceutical Science, Changsha, Hunan, 410013 China. Electronic address:
Article Synopsis
- The progression of Alcohol-associated liver disease (ALD) involves increased gut permeability due to ethanol, leading to bacterial products entering the bloodstream and causing liver inflammation and damage.
- The study used mice without the SIRT7 gene in myeloid cells, finding that this knockout reduced liver injury and inflammation caused by alcohol while minimally impacting lipid metabolism.
- Identification of CCL2 as a key target affected by SIRT7 highlights how its knockout hinders macrophage CCL2 secretion and monocyte recruitment, suggesting that targeting SIRT7 could provide new treatment strategies for ALD.
Regulation and Function of CCL2 and N-Myc in Retinoic Acid-treated Neuroblastoma Cells.
Cancer Genomics Proteomics
December 2024
Institute of Experimental and Clinical Pharmacology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany
Background/aim: Treatment with retinoic acid (RA) often promotes neuroblastoma differentiation and growth inhibition, including the suppression of the expression of the MYCN oncogene. However, RA also targets protumoral chemokines, such as CCL2, which may contribute to the development of resistance. The present study aimed to investigate the regulation and function of CCL2 and N-Myc in RA-treated neuroblastoma cells.
View Article and Find Full Text PDFEffect of imatinib on lipopolysaccharide‑induced acute lung injury and endothelial dysfunction through the P38 MAPK and NF-κB signaling pathways in vivo and in vitro.
Respir Physiol Neurobiol
December 2024
Department of Emergency Medicine, The Second Hospital of Tianjin Medical University, Tianjin 300211, China. Electronic address:
Background: The primary purpose of this study was to demonstrate the preventive effects of imatinib (IMA) on lipopolysaccharide (LPS)-induced inflammation in a mouse model of acute lung injury (ALI) and human umbilical vascular endothelial cells.
Methods: LPS stimulation for 24 h induced ALI and cell inflammation. The pathological results of the lungs were evaluated using the wet/dry weight ratio, pulmonary vascular permeability measurements, and myeloperoxidase immunohistochemistry.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!