AI Article Synopsis
- This study investigates the role of cancer stem-like cells and iPS-related genes in the development of colorectal cancer (CRC) and their impact on treatment response.
- Researchers analyzed tissue samples from 79 CRC patients to examine the expression of specific genes (c-MYC, SOX2, OCT3/4, LIN28, KLF4, and NANOG) related to stemness and their association with cancer progression.
- Key findings indicate that higher levels of LIN28 and SOX2 are linked to worse outcomes, such as lymph node metastasis and advanced Dukes stage, while KLF4 shows an inverse relationship with disease progression.
Article Abstract
Background: We previously determined that cancer stem-like cells may influence the susceptibility of colorectal cancer (CRC) cells to chemotherapeutic agents. Although Takahashi and Park identified a set of induced pluripotent stem cell (iPS)-related genes required for normal stem cell maintenance, the precise role of iPS-related gene expression in CRC pathogenesis remains to be determined. The purpose of this study was to clarify the clinical relevance of "stemness"-regulating gene expression in CRC cases.
Materials And Methods: Cancer cells were excised from tissues of 79 CRC cases by laser microdissection (LMD), and quantitative RT-PCR was used to evaluate expression levels of the iPS-related genes c-MYC, SOX2, OCT3/4, LIN28, KLF4, and NANOG, and to identify any associations between their expression and clinicopathological CRC progression.
Results: We found that LIN28 expression is significantly associated with lymph node metastasis (p = 0.018) and Dukes stage (p = 0.0319). SOX2expression is also correlated with lymph node metastasis. Furthermore, the ten cases with Dukes D disease expressed significantly higher levels of SOX2transcript than the other 69 cases (p = 0.0136). In contrast, KLF4 expression was inversely related to Dukes stage. Expression of c-MYC, OCT3/4, and NANOG did not appear to have clinical relevance in CRC cases.
Conclusion: The present analysis strongly suggests that altered expression of several iPS-related genes plays a role in CRC pathogenesis.
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| http://dx.doi.org/10.1245/s10434-009-0567-5 | DOI Listing |
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