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A Structural Model of Truncated Gaussia princeps Luciferase Elucidating the Crucial Catalytic Function of No.76 Arginine towards Coelenterazine Oxidation.
PLoS Comput Biol
January 2025
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, People's Republic of China.
Gaussia Luciferase (GLuc) is a renowned reporter protein that can catalyze the oxidation of coelenterazine (CTZ) and emit a bright light signal. GLuc comprises two consecutive repeats that form the enzyme body and a central putative catalytic cavity. However, deleting the C-terminal repeat only limited reduces the activity (over 30% residual luminescence intensity detectable), despite being a key part of the cavity.
View Article and Find Full Text PDFIn silico, in vitro and in vivo characterisation of thiamin binding proteins from plant seeds.
Biochem J
January 2025
Universiteit Gent, Ghent, Belgium.
Thiamin, an essential micronutrient, is a cofactor for enzymes involved in the central carbon metabolism and amino acids pathways. Despite efforts to enhance thiamin content in rice by incorporating thiamin biosynthetic genes, increasing thiamin content in endosperm remains challenging, possibly due to a lack of thiamin stability and/or a local sink. The introduction of storage proteins has been successful in biofortification strategies and similar efforts targeting thiamin led to a 3-4-fold increase in white rice.
View Article and Find Full Text PDFEffectiveness of nonsurgical re-instrumentation of residual pockets as step 3 of periodontal therapy: A field study.
J Periodontol
January 2025
Department of Periodontology, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Background: The study aims to analyze the effectiveness of nonsurgical re-instrumentation of residual pockets as step 3 of periodontal therapy and the stability of treatment outcomes at 18 months.
Methods: The study sample consisted of 489 patients diagnosed and treated nonsurgically for periodontitis. After a 6-month evaluation, residual pockets (≥5 mm) were re-instrumented.
The impact of straw and its post-pyrolysis incorporation on functional microbes and mineralization of organic carbon in yellow paddy soil.
PLoS One
January 2025
College of Agriculture, Guizhou University, Guiyang, China.
The impact of straw and biochar on carbon mineralization and the function of carbon cycle genes in paddy soil is important for soil nutrient management and the transformation of carbon pools. This research is based on a five-year field experiment with four treatments: no fertilizer application (CK); chemical fertilizer only (NPK); straw combined with chemical fertilizer (NPKS); and biochar combined with chemical fertilizer (NPKB). By integrating indoor mineralization culture with metagenomic approaches, we analyzed the response of organic carbon mineralization and carbon cycle genes in typical paddy soil from Guizhou Province, China, to different fertilization treatments.
View Article and Find Full Text PDFDiscovery of a Phosphodiesterase 7A Inhibitor of High Isozyme Selectivity Exhibiting Anti-Osteoporotic Effects.
ACS Med Chem Lett
January 2025
Chemical Research Laboratories, i2i-Labo, Biological Pharmacological Research Laboratories, and Drug Metabolism & Pharma-cokinetics Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco, Inc., 1-1, Murasaki-cho, Takatsuki, Osaka 569-1125, Japan.
Phosphodiesterases (PDEs) have drawn attention due to their critical roles in physiological and pathological conditions. Many research groups have studied these hydrolytic enzymes to develop new drugs, including apremilast as a PDE4 inhibitor and sildenafil as a PDE5 inhibitor. Targeting PDE7 has also been deemed a rational strategy to ameliorate autoimmune conditions.
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