AI Article Synopsis
- Researchers created a mouse model called MIP-Luc-VU that allows for non-invasive tracking of beta cell mass changes using bioluminescence.* -
- The study showed that diabetic mice with reduced beta cell mass had decreasing bioluminescence, while those on a high-fat diet with increased beta cell mass showed more bioluminescence.* -
- This mouse model can effectively measure beta cell mass over long periods, especially after islet transplantation, indicating its potential for future diabetes research.*
Article Abstract
Purpose: We developed a mouse model that enables non-invasive assessment of changes in beta cell mass.
Procedures: We generated a transgenic mouse expressing luciferase under control of the mouse insulin I promoter [mouse insulin promoter-luciferase-Vanderbilt University (MIP-Luc-VU)] and characterized this model in mice with increased or decreased beta cell mass and after islet transplantation.
Results: Streptozotocin-induced, diabetic MIP-Luc-VU mice had a progressive decline in bioluminescence that correlated with a decrease in beta cell mass. MIP-Luc-VU animals fed a high-fat diet displayed a progressive increase in bioluminescence that reflected an increase in beta cell mass. MIP-Luc-VU islets transplanted beneath the renal capsule or into the liver emitted bioluminescence proportional to the number of islets transplanted and could be imaged for more than a year.
Conclusions: Bioluminescence in the MIP-Luc-VU mouse model is proportional to beta cell mass in the setting of increased and decreased beta cell mass and after transplantation.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822006 | PMC |
| http://dx.doi.org/10.1007/s11307-009-0240-1 | DOI Listing |
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