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Integrating polyurethane culture substrates into poly(dimethylsiloxane) microdevices. | LitMetric

Integrating polyurethane culture substrates into poly(dimethylsiloxane) microdevices.

Biomaterials

Department of Mechanical and Industrial Engineering, University of Toronto, 5 King's College Road, Toronto, Ontario M5S 3G8, Canada.

Published: October 2009

AI Article Synopsis

  • PDMS-based microdevices are excellent for quick assessments of cellular responses to controlled environmental factors but are not suitable for long-term cell cultures due to their effects on cell behavior.
  • This study introduces a method to incorporate polyurethane (PU) into PDMS microfabrication, allowing for long-term cell culture on alternative substrates.
  • Results show that while initial cell adhesion and spreading are similar on PU and collagen-coated PDMS, PU provides improved cell maintenance over longer durations compared to PDMS, making it a better option for extended experiments in microdevice-based research.

Article Abstract

Poly(dimethylsiloxane) (PDMS)-based microdevices have enabled rapid, high-throughput assessment of cellular response to precisely controlled microenvironmental stimuli, including chemical, matrix and mechanical factors. However, the use of PDMS as a culture substrate precludes long-term culture and may significantly impact cell response. Here we describe a method to integrate polyurethane (PU), a well-studied and clinically relevant biomaterial, into the PDMS multilayer microfabrication process, enabling the exploration of long-term cellular response on alternative substrates in microdevices. To demonstrate the utility of these hybrid microdevices for cell culture, we compared initial cell adhesion, cell spreading, and maintenance of protein patterns on PU and PDMS substrates. Initial cell adhesion and cell spreading after three days were comparable between collagen-coated PDMS and PU substrates (with or without collagen coating), but significantly lower on native PDMS substrates. However, for longer culture durations (> or = 6 days), cell spreading and protein adhesion on PU substrates was significantly better than that on PDMS substrates, and comparable to that on tissue culture-treated polystyrene. Thus, the use of a generic polyurethane substrate in microdevices enables longer-term cell culture than is possible with PDMS substrates. More generally, this technique can improve the impact and applicability of microdevice-based research by facilitating the use of alternate, relevant biomaterials while maintaining the advantages of using PDMS for microdevice fabrication.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biomaterials.2009.05.066DOI Listing

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