Using heavy-ion microbeam, we report target irradiation of selected compartments within the diapause-terminated egg and its mutational consequences in the silkworm, Bombyx mori. On one hand, carbon-ion exposure of embryo to 0.5-6 Gy increased the somatic mutation frequency, suggesting targeted radiation effects. On the other, such increases were not observed when yolk was targeted, suggesting a lack of nontargeted bystander effect.
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http://dx.doi.org/10.1269/jrr.09021 | DOI Listing |
Biology (Basel)
October 2024
Department of Quantum-Applied Biosciences, Takasaki Institute for Advanced Quantum Science (TIAQS), National Institutes for Quantum Science and Technology (QST), 1233 Watanuki, Takasaki 370-1292, Gunma, Japan.
J Radiat Res
September 2024
Institute of Modern Physics, Fudan University, Shanghai 200433, China.
A Monte Carlo simulation was used to assess the performance of a collimated hollow X-ray microbeam for subcellular cytoplasm irradiation. A high-Z coaxial collimation structure with an inner core for nucleus shielding was investigated. Two key performances, the extraction efficiency (cytoplasm dose per unit incident fluence) and the dose contrast (cytoplasm-to-nucleus dose ratio), were evaluated regarding the influences of the material, geometry and physical arrangements of the collimator, target dish and incident beam source.
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February 2024
Institute for Applied Physics and Measurement Technology, Universität der Bundeswehr München, Neubiberg, Germany.
High-linear energy transfer (LET) radiation, such as heavy ions is associated with a higher relative biological effectiveness (RBE) than low-LET radiation, such as photons. Irradiation with low- and high-LET particles differ in the interaction with the cellular matter and therefore in the spatial dose distribution. When a single high-LET particle interacts with matter, it results in doses of up to thousands of gray (Gy) locally concentrated around the ion trajectory, whereas the mean dose averaged over the target, such as a cell nucleus is only in the range of a Gy.
View Article and Find Full Text PDFBiology (Basel)
December 2023
Takasaki Institute for Advanced Quantum Science (TIAQ), National Institutes for Quantum Science and Technology (QST), Gunma 370-1292, Japan.
The functions of organisms are performed by various tissues composed of different cell types. Localized irradiation with heavy-ion microbeams, which inactivate only a portion of the constituent cells without destroying the physical intercellular connections of the tissue, is a practical approach for elucidating tissue functions. However, conventional collimated microbeams are limited in the shape of the area that can be irradiated.
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