Using heavy-ion microbeam, we report target irradiation of selected compartments within the diapause-terminated egg and its mutational consequences in the silkworm, Bombyx mori. On one hand, carbon-ion exposure of embryo to 0.5-6 Gy increased the somatic mutation frequency, suggesting targeted radiation effects. On the other, such increases were not observed when yolk was targeted, suggesting a lack of nontargeted bystander effect.

Download full-text PDF

Source
http://dx.doi.org/10.1269/jrr.09021DOI Listing

Publication Analysis

Top Keywords

heavy-ion microbeam
8
diapause-terminated egg
8
silkworm bombyx
8
bombyx mori
8
somatic mutation
8
targeted heavy-ion
4
microbeam irradiation
4
irradiation embryo
4
embryo yolk
4
yolk diapause-terminated
4

Similar Publications

Advances in Targeted Microbeam Irradiation Methods for Live .

Biology (Basel)

October 2024

Department of Quantum-Applied Biosciences, Takasaki Institute for Advanced Quantum Science (TIAQS), National Institutes for Quantum Science and Technology (QST), 1233 Watanuki, Takasaki 370-1292, Gunma, Japan.

Article Synopsis
  • Charged-particle microbeam irradiation devices allow for targeted irradiation of individual cells and tissues using heavy-ion or proton beams, with applications seen in Japan, the United States, China, and France.
  • This technology is particularly useful for studying the effects of irradiation on mammalian cancer cells, focusing on bystander effects.
  • The paper reviews advancements in microbeam biology, particularly since the first individual-level microbeam irradiation of nematodes in 2006, while addressing unique challenges related to immobilizing subjects for experiments.
View Article and Find Full Text PDF

A Monte Carlo simulation was used to assess the performance of a collimated hollow X-ray microbeam for subcellular cytoplasm irradiation. A high-Z coaxial collimation structure with an inner core for nucleus shielding was investigated. Two key performances, the extraction efficiency (cytoplasm dose per unit incident fluence) and the dose contrast (cytoplasm-to-nucleus dose ratio), were evaluated regarding the influences of the material, geometry and physical arrangements of the collimator, target dish and incident beam source.

View Article and Find Full Text PDF
Article Synopsis
  • Radiotherapy is an important cancer treatment, but research in low-income countries has some tough problems.
  • A 2022 survey provided new information on how these countries are dealing with radiotherapy and the funding they get.
  • The paper talks about new technology and ideas that can help improve cancer treatment in places that struggle with resources and emphasizes the need for more trained researchers in this field.
View Article and Find Full Text PDF

Enhanced RBE of Particle Radiation Depends on Beam Size in the Micrometer Range.

Radiat Res

February 2024

Institute for Applied Physics and Measurement Technology, Universität der Bundeswehr München, Neubiberg, Germany.

High-linear energy transfer (LET) radiation, such as heavy ions is associated with a higher relative biological effectiveness (RBE) than low-LET radiation, such as photons. Irradiation with low- and high-LET particles differ in the interaction with the cellular matter and therefore in the spatial dose distribution. When a single high-LET particle interacts with matter, it results in doses of up to thousands of gray (Gy) locally concentrated around the ion trajectory, whereas the mean dose averaged over the target, such as a cell nucleus is only in the range of a Gy.

View Article and Find Full Text PDF

The functions of organisms are performed by various tissues composed of different cell types. Localized irradiation with heavy-ion microbeams, which inactivate only a portion of the constituent cells without destroying the physical intercellular connections of the tissue, is a practical approach for elucidating tissue functions. However, conventional collimated microbeams are limited in the shape of the area that can be irradiated.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!