PCR amplification of target genes from environmental DNA extracts can suffer from PCR inhibition, caused by co-extracted substances. No simple assay has been available to quantify this inhibition. Therefore, a generally applicable PCR inhibition-assay was developed, which allows determination of statistically significant inhibition of PCR. This information is important when documenting quality of DNA in environmental extracts used as template for PCR.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mimet.2009.06.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Initial Psychometric Testing of the Harm Reduction Self-Efficacy Scale (HRSES).
Addict Res Theory
November 2023
Center on Alcohol, Substance use, And Addictions (CASAA), University of New Mexico.
Abstinence self-efficacy, belief in one's ability to abstain, has been identified as a predictor of substance use behavior change. Yet, many people who use substances do not want to abstain. Self-efficacy for achieving a range of goals (i.
View Article and Find Full Text PDFFirst-principles predictions of two-dimensional Ce-based ferromagnetic semiconductors: CeF and CeFCl monolayers.
RSC Adv
January 2025
School of Electronic Information, Huzhou College Huzhou 313000 China
Two-dimensional (2D) ferromagnetic (FM) semiconductors hold great promise for the next generation spintronics devices. By performing density functional theory first-principles calculations, both CeF and CeFCl monolayers are studied, our calculation results show that CeF is a FM semiconductor with sizable magneto-crystalline anisotropy energy (MAE) and high Curie temperature (290 K), but a smaller band gap and thermal instability indicate that it is not applicable at higher temperature. Its isoelectronic analogue, the CeFCl monolayer, is a bipolar FM semiconductor, its dynamics, elastic, and thermal stability are confirmed, our results demonstrate promising applications of the CeFCl monolayer for next-generation spintronic devices owing to its high Curie temperature (200 K), stable semiconducting features, and stability.
View Article and Find Full Text PDFMacrophage Polarisation in the Tumour Microenvironment: Recent Research Advances and Therapeutic Potential of Different Macrophage Reprogramming.
Cancer Control
January 2025
Department of Thoracic Surgery, Affiliated Hospital 6 of Nantong University, Medical School of Nantong University, Nantong, PR China.
Background: Macrophages are a critical component of the innate immune system, derived from monocytes, with significant roles in anti-inflammatory and anti-tumour activities. In the tumour microenvironment, however, macrophages are often reprogrammed into tumour-associated macrophages (TAMs), which promote tumour growth, metastasis, and therapeutic resistance.
Purpose: To review recent advancements in the understanding of macrophage polarisation and reprogramming, highlighting their role in tumour progression and potential as therapeutic targets.
Mechanically Triggered Protein Desulfurization.
J Am Chem Soc
January 2025
New Cornerstone Science Laboratory, Tsinghua-Peking Joint Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.
The technology of native chemical ligation and postligation desulfurization has greatly expanded the scope of modern chemical protein synthesis. Here, we report that ultrasonic energy can trigger robust and clean protein desulfurization, and we developed an ultrasound-induced desulfurization (USID) strategy that is simple to use and generally applicable to peptides and proteins. The USID strategy involves a simple ultrasonic cleaning bath and an easy-to-use and easy-to-remove sonosensitizer, titanium dioxide.
View Article and Find Full Text PDFDeep learning enabled rapid classification of yeast species in food by imaging of yeast microcolonies.
Food Res Int
February 2025
Department of Food Science & Technology, University of California-Davis, Davis, CA 95616, USA; Department of Biological & Agricultural Engineering, University of California-Davis, Davis, CA 95616, USA. Electronic address:
Diverse species of yeasts are commonly associated with food and food production environments. The contamination of food products by spoilage yeasts poses significant challenges, leading to quality degradation and food loss. Similarly, the introduction of undesirable strains during fermentation can cause considerable challenges with the quality and progress of the fermentation process.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!