AI Article Synopsis
- - A new high-performance anion-exchange chromatography method with pulsed amperometric detection was created for the quick detection of phenylalanine (Phe) to help diagnose phenylketonuria (PKU).
- - The method is efficient, with sample pretreatment made easier and a separation time of just 5 minutes, alongside a detection limit of 50 pg for Phe.
- - It showed excellent accuracy, differentiating between PKU-positive individuals and healthy individuals, making it a reliable option for rapid screening and ongoing monitoring of PKU.
Article Abstract
We have developed a high-performance anion-exchange chromatography with pulsed amperometric detection method for the detection of phenylalanine (Phe) and diagnosis of phenylketonuria (PKU). Sample pretreatment steps were simplified without derivatization. The analyte was separated within 5 min. The detection limit (S/N=3) for Phe was 50 pg. Linear dynamic range was 1.23-14.43 mg/dL (r(2)=0.9999) for a dried blood spot. The mean recoveries of Phe for intra- and inter-day assays were found to be 96.87-104.16%. This method clearly differentiated PKU-positive groups from normal groups, and proved to be a practical procedure for rapid screening and follow-up monitoring of PKU.
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| http://dx.doi.org/10.1016/j.chroma.2009.06.004 | DOI Listing |
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