It has been suggested that copper-zinc superoxide dismutase (CuZnSOD) isoforms of distinct isoelectric point (pI) could result from differences in their metallation state. Our aim was then to develop and validate analytical methods for the determination and understanding of metallation states in human CuZnSOD isoforms. To avoid metal losses during sample preparation steps, CuZnSOD isoforms were separated according to their pI using non-denaturing isoelectric focusing (IEF) gel electrophoresis. Metal quantification was directly performed in-gel. Cu/Zn ratios of CuZnSOD isoforms were quantified by Particle-Induced X-ray Emission (PIXE) and Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS). Cu/Zn ratios were measured close to the value of 1 as expected from the known stoichiometry of CuZnSOD with slight, but statistically significant, differences between acidic and basic isoforms. Overall, this study demonstrates that metal quantification can be performed directly on metalloproteins separated on electrophoresis gels.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.biochi.2009.05.016 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evolutionary Conserved and Divergent Responses to Copper Zinc Superoxide Dismutase Inhibition in Plants.
Plant Cell Environ
October 2024
Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany.
Article Synopsis
- Life evolved in a reducing environment but faced challenges from reactive oxygen species (ROS) during the great oxidation event (GOE), leading to the development of copper-zinc superoxide dismutases (CuZnSODs) in some plants.
- The chemical inhibitor lung cancer screen 1 (LCS-1) was used to study the effects of CuZnSOD inhibition on plant growth, transcription, and metabolism across different species, including bryophytes and vascular plants.
- The results showed that LCS-1 caused oxidative stress and a core physiological response related to glutathione balance in all species, but varying metabolic responses were observed based on the number and types of CuZnSOD isoforms present in each plant.
Liver Oxidative Status, Serum Lipids Levels after Bariatric Surgery and High-Fat, High-Sugar Diet in Animal Model of Induced Obesity.
Int J Mol Sci
November 2023
Department of Physiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana Street, 41-808 Zabrze, Poland.
Nutritional status is a major determinant of hepatocyte injuries associated with changed metabolism and oxidative stress. This study aimed to determine the relations between oxidative stress, bariatric surgery, and a high-fat/high-sugar (HFS) diet in a diet-induced obesity rat model. Male rats were maintained on a control diet (CD) or high-fat/high-sugar diet (HFS) inducing obesity.
View Article and Find Full Text PDFCuZn-superoxide dismutase is differentially modified in localization and expression by three abiotic stresses in miniature rose bushes.
Micron
November 2023
ImageUP, Plate-forme d'imagerie de l'Université de Poitiers, Pôle Biologie Santé, 1 rue Georges Bonnet, Bât. B37, TSA 51106, 86073 Poitiers Cedex 9, France. Electronic address:
Three abiotic stresses, copper application (CS), mechanical rubbing (MS) and water deprivation (WS) applied on miniature rose bushes specifically activate the expression of the CuZn-Superoxide dismutase (SOD). The Cu/Zn-SOD protein immunodetected in the 4th internode was shown engaged in lignification in phloem, cambium and xylem cells. The SOD occurrence was detailed in the vessel associated cells (VACs), using immunogold labeling observed in transmission electron microscopy.
View Article and Find Full Text PDFEvaluation of antioxidant parameters of multiple sclerosis patients' serum according to the disease course.
Mult Scler Relat Disord
September 2023
Department of Neurology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland.
Introduction: Multiple sclerosis (MS) is a chronic, demyelinating disease of the central nervous system. Its clinical courses are clinically isolated syndrome (CIS), relapsing remitting (RRMS), secondary progressive (SPMS), and primary progressive (PPMS). The differentiation of MS types is crucial for adequate treatment.
View Article and Find Full Text PDFB" and C subunits of PP2A regulate the levels of reactive oxygen species and superoxide dismutase activities in Arabidopsis.
Plant Physiol Biochem
February 2023
Department of Botany, Faculty of Science and Technology, University of Debrecen, Hungary. Electronic address:
The serine-threonine protein phosphatases PP2A regulate many cellular processes, however their role in oxidative stress responses and defence is less known. We show the involvement of its C (catalytic) and B" (a regulatory) subunits. The c3c4 (C subunit) and fass (B") subunit mutants and Col wt of Arabidopsis were used.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!