AI Article Synopsis
- New experimental data on the viscous fingering (VF) process were gathered in ordered pillar array columns, providing a different perspective compared to traditional packed bed columns.
- A fluorescence microscope with a CCD camera enabled detailed quantitative analysis of how VF contributes to band broadening during the study.
- The study found that the plate height increase due to VF varies significantly based on viscosity contrast and injection volume, highlighting differences in results from 0.5 microm to 20 microm depending on the conditions tested.
Article Abstract
New experimental data of the viscous fingering (VF) process have been generated by studying the VF process in perfectly ordered pillar array columns instead of in the traditionally employed packed bed columns. A detailed quantitative analysis of the contribution of VF to the observed band broadening could be made by following the injected species bands using a fluorescence microscope equipped with a CCD-camera. For a viscosity contrast of 0.16 cP, a plate height increase of about 1 microm can be observed, while for a contrast of respectively 0.5 cP and 1 cP, additional plate height contributions of the order of 5-20 microm were observed. Citing these values is however futile without noting that they also depend extremely strongly on the injection volume of injected sample. It was found that, for a given viscosity contrast of 0.314 cP, the maximal plate height increase varied between 0.5 microm and 18 microm if the injection volume was varied between 3.0 nl and 32.7 nl. These values furthermore also strongly vary with the distance along the column axis.
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| http://dx.doi.org/10.1016/j.chroma.2009.04.098 | DOI Listing |
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