Background: The mollicute Mycoplasma conjunctivae is the etiological agent leading to infectious keratoconjunctivitis (IKC) in domestic sheep and wild caprinae. Although this pathogen is relatively benign for domestic animals treated by antibiotics, it can lead wild animals to blindness and death. This is a major cause of death in the protected species in the Alps (e.g., Capra ibex, Rupicapra rupicapra).
Methods: The genome was sequenced using a combined technique of GS-FLX (454) and Sanger sequencing, and annotated by an automatic pipeline that we designed using several tools interconnected via PERL scripts. The resulting annotations are stored in a MySQL database.
Results: The annotated sequence is deposited in the EMBL database (FM864216) and uploaded into the mollicutes database MolliGen http://cbi.labri.fr/outils/molligen/ allowing for comparative genomics.
Conclusion: We show that our automatic pipeline allows for annotating a complete mycoplasma genome and present several examples of analysis in search for biological targets (e.g., pathogenic proteins).
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2697654 | PMC |
| http://dx.doi.org/10.1186/1471-2105-10-S6-S7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Different Types of Co-Infection by Contagious Ecthyma Virus, Enteropathogenic , , Ecto- and Endo-Parasites in Four Young Alpine Ibex ().
Animals (Basel)
December 2024
Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Regione Amerique 7G, 11020 Quart, AO, Italy.
The research aimed to investigate the perinatal pathology of Alpine ibex () through the study of four young subjects (at the age of 3 to 4 months) found dead in Valle d'Aosta, a region of northwestern Italy. The carcasses were submitted to necropsy followed by an examination of ecto- and endoparasites (ECP and ENP); samples from the gross lesions (in summary, cutaneous papilloma and crusts, ocular discharge, lobular haemorrhagic areas in the lungs, catarrhal-haemorrhagic enterocolitis) were analysed by bacteriological, histopathological, and biomolecular methods to define the etiological agent. The subjects, with various co-infection patterns, were affected by contagious ecthyma virus (ORFV) (agent of a highly diffusive pustular dermatitis transmissible to small ruminants and humans), Enteropathogenic (EPEC) (major etiological agent of infantile diarrhoea especially in developing countries), (MC) (cause of an ocular infection common to goats and sheep), various ECP (ticks and keds) and ENP (lung and intestinal nematodes, and coccidia).
View Article and Find Full Text PDFCase-control study to identify the causative agents of ophthalmia and conjunctivitis in goats in Savannakhet province of Lao PDR.
Vet Microbiol
September 2024
School of Environmental and Rural Science, University of New England, Armidale, NSW, Australia.
Pinkeye is a highly contagious disease of goats with different aetiologies. Surveys in Lao PDR have identified eye lesions typical of pinkeye as a common condition, however, this has not been confirmed diagnostically, and the responsible pathogens have not been identified. A matched case-control study was implemented in 70 goat holdings from Savannakhet province, Lao PDR, to detect agents causing pinkeye and conduct phylogenetic analysis of the identified pathogens.
View Article and Find Full Text PDFWildlife-Livestock Host Community Maintains Simultaneous Epidemiologic Cycles of in a Mountain Ecosystem.
Vet Sci
May 2024
Parque Nacional y Parque Natural de Sierra Nevada and Wildlife Ecology & Health Group (WE&H), Pinos Genil, E-18191 Granada, Spain.
Infectious keratoconjunctivitis (IKC) is an eye disease caused by that affects domestic and wild caprines, including Iberian ibex (), a medium-sized mountain ungulate. However, its role in IKC dynamics in multi-host communities has been poorly studied. This study assessed in Iberian ibex and seasonally sympatric domestic small ruminants in the Natural Space of Sierra Nevada (NSSN), a mountain habitat in southern Spain.
View Article and Find Full Text PDFBacteria associated with infectious keratoconjunctivitis outbreak in confined lambs.
J Med Microbiol
May 2022
RuminAção - Teaching, Research and Extension in Ruminants, School of Veterinary, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Infectious keratoconjunctivitis is a significant ocular disease found in confined sheep. Little information about the aetiological agents and their antimicrobial susceptibility is available. There is limited information on the aetiological agents involved in keratoconjunctivitis outbreaks in sheep.
View Article and Find Full Text PDFIdentification of Mycoplasma species and related organisms from ruminants in England and Wales during 2005-2019.
BMC Vet Res
October 2021
Mycoplasma Team, Department of Bacteriology, Animal and Plant Health Agency, Weybridge, Surrey, UK.
Background: Mycoplasma species have been associated with economically important diseases affecting ruminants worldwide and include contagious bovine pleuropneumonia (CBPP), contagious caprine pleuropneumonia (CCPP) and contagious agalactia, listed by the World Organisation for Animal Health (OIE). The Mycoplasma Team at the Animal and Plant Health Agency provides an identification service for Mycoplasma and Ureaplasma species of veterinary importance to the United Kingdom (UK), supporting the detection of new and emerging pathogens, as well as contributing to the surveillance of endemic, and the OIE listed diseases exotic to the UK. Mycoplasma and other Mollicutes species were identified from diagnostic samples from farmed ruminants in England and Wales using a combination of culture and 16S rRNA gene-based PCR-denaturing gradient gel electrophoresis, submitted between 2005 and 2019.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!