Strain 344 synthesizing an antibiotic complex was isolated after fusion of the protoplasts of Streptomyces monomycini producing monomycin and Streptomyces kanamyceticus producing kanamycin. The major component of the complex was identified with albofungin and the minor one was suggested to be chloralbofungin. In the cultures of strain 344 variants forming monomycin were detected. After regeneration of the protoplasts of the parent strains there were isolated no stable clones synthesizing antibiotics differing from monomycin and kanamycin.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Construction of a fusant bacterial strain simultaneously degrading atrazine and acetochlor and its application in soil bioremediation.
Sci Total Environ
January 2025
Jilin Academy of Agricultural Sciences (Northeast Agricultural Research Center of Chin), Gongzhuling 136100, Jilin, China. Electronic address:
Application of herbicide-degrading bacteria is an effective strategy to remove herbicide in soil. However, the ability of bacteria to degrade a herbicide is often severely limited in the presence of other pesticide. In this study, the atrazine-degrading strain Klebsiella varicola FH-1 and acetochlor-degrading strain Bacillus Aryabhatti LY-4 were used as parent strains to construct the recombinant RH-92 strain through protoplast fusion technology.
View Article and Find Full Text PDFDevelopment of an optimized protocol for protoplast-to-plant regeneration of selected varieties of Brassica oleracea L.
BMC Plant Biol
December 2024
Department of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculturein Krakow, Mickiewicza 21, Krakow, 31-120, Poland.
Background: Brassica oleracea L. is a key plant in the Brassicaceae family, known for popular vegetables like cabbage, broccoli, kale and collard. Collard (B.
View Article and Find Full Text PDFImprovement of silage characteristics of HMC4 and improvement of silage quality of king grass.
Front Microbiol
December 2024
Zhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China.
The effect of HMC4 produced by protoplast fusion on silage was studied. The silage formula was composed of heterozygote HMC4 (Group C), parent Lactobacillus (Group A) and a combination of two parents (Group B). The fermentation quality and microbial composition of each batch of silage were evaluated.
View Article and Find Full Text PDFOptimizing L. Breeding Through Somatic Hybridization Using Cytoplasmic Male Sterility (CMS) Lines: From Protoplast Isolation to Plantlet Regeneration.
Plants (Basel)
November 2024
Department of Biotechnology, Genomic and Plant Breeding, Institute for Agroenvironmental Research and Development of Murcia (IMIDA), c/Mayor s/n, E-30150 Murcia, Spain.
The L. species embrace important horticultural crops, such as broccoli, cauliflower, and cabbage, which are highly valued for their beneficial nutritional effects. However, the complexity of flower emasculation in these species has forced breeders to adopt biotechnological approaches such as somatic hybridization to ease hybrid seed production.
View Article and Find Full Text PDFOptimization of ultrasound-mediated DNA transfer for bacteria and preservation of frozen competent cells.
Microbiol Spectr
November 2024
Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, China.
Unlabelled: The transformation of DNA into cells is the basis of molecular biology. Commonly employed techniques include heat shock transformation, electro-transformation, conjugation, transduction, and protoplast fusion. Recently, ultrasonic transformation technology has been developed to transfer DNA into competent cells.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!