Experimental mathematical designs were applied for optimization of a nutrient medium for biosynthesis of the antifungal antibiotic AK-111-81 by phosphate-deregulated mutant of Streptomyces hygroscopicus 111-81. Antifungal antibiotic AK-111-81 possesses well-expressed activity against Fusarium graminearum and other phytopathogenic fungi. The level of the production of the antibiotic AK-111-81 on this medium is more than three times higher than on the initial medium. The optimized quantitative composition of the nutrient culture media is (g/l): glucose, 20; soy meal, 18; ammonium succinate, 3; CaCO(3), 1.
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Optimization of the production medium for biosynthesis of antifungal antibiotic AK-111-81 by phosphate-deregulated mutant of Streptomyces hygroscopicus.
Appl Biochem Biotechnol
July 2009
Institute of Microbiolgy, Bulgarian Academy of Sciences, Department of Microbial Biosynthesis and Biotechnology, Sofia, Bulgaria.
Experimental mathematical designs were applied for optimization of a nutrient medium for biosynthesis of the antifungal antibiotic AK-111-81 by phosphate-deregulated mutant of Streptomyces hygroscopicus 111-81. Antifungal antibiotic AK-111-81 possesses well-expressed activity against Fusarium graminearum and other phytopathogenic fungi. The level of the production of the antibiotic AK-111-81 on this medium is more than three times higher than on the initial medium.
View Article and Find Full Text PDFSpontaneous white sectored-mutants in Streptomyces hygroscopicus 111-81: characterization and antibiotic productivity.
Curr Microbiol
August 2008
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
Spontaneous white mutants from sectors of Streptomyces hygroscopicus 111-81 were isolated. The comparison of morphological, cultural, and biochemical properties of the mutants and ancestor showed the differences in colors of aerial, substrate mycelia, and sporulation. Changes in resistance to antibiotics and sensitivity to lysozyme indicated alterations in cell walls and cell membranes of the mutants.
View Article and Find Full Text PDFEffects of nutrients on the production of AK-111-81 macrolide antibiotic by Streptomyces hygroscopicus.
Microbiol Res
August 2005
Institute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.
The influence of different carbon and nitrogen sources on the production of AK-111-81 nonpolyenic macrolide antibiotic by Streptomyces hygroscopicus 111-81 was studied. Substitution of glucose with lactose or glycerol significantly affected maximal antibiotic AK-111-81 productivity as the growth rate was close to that of the basal fermentation medium. Addition of ammonium succinate to the fermentation medium markedly increased the antibiotic productivity as the growth rate was low.
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