Fluorescence intensity of thioflavin T fluorogenic label increased significantly as a result of formation of enzyme-inhibitory complex with acetylcholinesterase of human blood erythrocytes. Thioflavin T is a reversible inhibitor, selectively reacting with acetylcholinesterase. Thioflavin T fluorescence intensity is proportional to acetylcholinesterase activity for the studied interval of enzyme activities. A rapid fluorescent method for measuring acetylcholinesterase activity is proposed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10517-009-0436-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Gold nanoclusters/manganese dioxide nanosheets hybrid nanozyme with fluorescence and oxidase-like activity for dual-mode detection of acetylcholinesterase and inhibitors screening.
Anal Bioanal Chem
December 2024
College of Pharmacy, Key Laboratory of Innovative Drug Development and Evaluation, Hebei Medical University, Shijiazhuang, 050017, China.
The abnormal expression of acetylcholinesterase (AChE) is linked to the development of various diseases. Accurate determination of AChE activity as well as screening AChE inhibitors (AChEIs) holds paramount importance for early diagnosis and treatment of AChE-related diseases. Herein, a fluorescent and colorimetric dual-channel probe based on gold nanoclusters (AuNCs) and manganese dioxide nanosheets (MnO NSs) was developed.
View Article and Find Full Text PDFA hybrid artificial neural network and multi-objective genetic algorithm approach to optimize extraction conditions of Mentha longifolia and biological activities.
Sci Rep
December 2024
Department of Pharmacy Services, Vocational School of Health Services, Osmaniye Korkut Ata University, Osmaniye, Turkey.
In this work, artificial neural network coupled with multi-objective genetic algorithm (ANN-NSGA-II) has been used to develop a model and optimize the conditions for the extracting of the Mentha longifolia (L.) L. plant.
View Article and Find Full Text PDFTiCT MXene nanoribbons@MnO: A novel multifunctional probe for colorimetric and fluorescence dual-response sensing of trichlorfon.
Talanta
December 2024
Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China; College of Agricultural Equipment Engineering, Henan University of Science and Technology, Luoyang, 471003, China. Electronic address:
Manganese dioxide nanosheets (MnO NSs) have garnered significant attention in analytical sensing, while the majority of the previous reports suffer from a complex preparation process involving reducing agents, template or high-temperature. In this work, a novel MnO NSs decorated TiCT MXene nanoribbons (TiCTNR@MnO) composite was firstly assemblied via a facile one-step strategy and applied as a bi-signal generator to enable colorimetric and fluorescence (FL) dual-response sensing. During the assembly process, TiCTNR innovatively acted as both reductant and carrier to prevent the aggregation of MnO NSs.
View Article and Find Full Text PDFDiscovery of novel bicyclic and tricyclic cyclohepta[b]thiophene derivatives as multipotent AChE and BChE inhibitors, in-Vivo and in-Vitro assays, ADMET and molecular docking simulation.
Eur J Med Chem
December 2024
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sinai University, Kantara Branch, Ismailia, 41636, Egypt.
Alzheimer's disease (AD) is primarily caused by oxidative stress, hyperphosphorylated τ-protein aggregation, and amyloid-β deposition. Changes in dopaminergic and serotoninergic neurotransmitter pathways are linked to certain symptoms of AD. Derivatives of bicyclic and tricyclic cyclohepta[b]thiophene were developed to identify new potential candidates as acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitors for the treatment of AD.
View Article and Find Full Text PDFDeoxyvasicinone hybrids in the management of Alzheimer's disease: Recent advances on manmade derivatives, pharmacological activities, and structure-activity relationship.
Arch Pharm (Weinheim)
January 2025
Department of Pharmacognosy, University Institute of Pharma Sciences, Chandigarh University, Mohali, Punjab, India.
Alzheimer's disease (AD) is a prevalent neurological illness that affects over 80% of aged adults globally in cases of dementia. Although the exact pathophysiological causes of AD remain unclear, its pathogenesis is primarily driven by several distinct biochemical alterations: (i) the accumulation of toxic Aβ plaques, (ii) the hyperphosphorylation of tau proteins, (iii) oxidative stress resulting in cell death, and (iv) an imbalance between the two main neurotransmitters, glutamate and acetylcholine (ACh). Currently, there are very few medications available and no treatment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!