Statistical distributions of sequencing by synthesis with probabilistic nucleotide incorporation.

Sequencing by synthesis is used in many next-generation DNA sequencing technologies. Some of the technologies, especially those exploring the principle of single-molecule sequencing, allow incomplete nucleotide incorporation in each cycle. We derive statistical distributions for sequencing by synthesis by taking into account the possibility that nucleotide incorporation may not be complete in each flow cycle. The statistical distributions are expressed in terms of nucleotide probabilities of the target sequences and the nucleotide incorporation probabilities for each nucleotide. We give exact distributions both for fixed number of flow cycles and for fixed sequence length. Explicit formulas are derived for the mean and variance of these distributions. The results are generalizations of our previous work for pyrosequencing. Incomplete nucleotide incorporation leads to significant change in the mean and variance of the distributions, but still they can be approximated by normal distributions with the same mean and variance. The results are also generalized to handle sequence context dependent incorporation. The statistical distributions will be useful for instrument and software development for sequencing by synthesis platforms.