Objective: To make clear the molecular pathways involved in hydrogen peroxide-induced spiral ganglion cells death.
Method: The spiral ganglion cells of the newly born rats were primary cultured. Then the SGCs were exposed to hydrogen peroxide for different concentrations (0, 100, 200, 500 micromol/L) and for different hours (2, 4, 6 h). Cell nucleic were stained simultaneously with the DNA binding dyes Hoechst 33258 and propidium iodide.
Result: At lower concentrations of hydrogen peroxide, apoptosis was the main reason for cell death. At higher concentrations of hydrogen peroxide, the cells died mainly by necrosis.
Conclusion: The effects of hydrogen peroxide are dose and time dependency. Reactive oxygen species may play a role as an early molecule signal in the mechanism of SGCs death.
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